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α sp1

Manufactured by Cell Signaling Technology
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The α-SP1 is a primary antibody that specifically recognizes the Sp1 transcription factor. Sp1 is a zinc finger protein that binds to GC-rich motifs and regulates the expression of numerous genes involved in a variety of cellular processes.

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Lab products found in correlation

α pchk2, by Cell Signaling Technology (1 mentions) α akt, by Cell Signaling Technology (1 mentions) α egfr, by Cell Signaling Technology (1 mentions) α fak, by Cell Signaling Technology (1 mentions) α mek, by Cell Signaling Technology (1 mentions) α perk1 2, by Cell Signaling Technology (1 mentions) α erk1 2, by Cell Signaling Technology (1 mentions) α β catenin, by Cell Signaling Technology (1 mentions) α tubulin, by Cell Signaling Technology (1 mentions) Leupeptin, by Merck Group (1 mentions) α tubulin, by Abcam (1 mentions)

2 protocols using α sp1

1

Western Blot Analysis of Cell Signaling

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The cells were homogenized in lysis buffer (50 mM/L HEPES pH 7.5, 150 mM/L NaCl, 10% glycerol, 1% Triton X-100, 1 mM/L EGTA, 1.5 mM/L MgCl2, 10 mM/L NaF, 10 mM/L sodium pyrophosphate, 1mM/L Na3VO4, 10 μg approtinin/ml, 10 μg leupeptin/ml) (Sigma). The following antibodies were used: α-RAS, α-RAC, α-CDC42, α-RHO, α-pEGFR, α-EGFR, α-pFAK, α-FAK, α-pSCR, α-SCR, α-pMEK, α-MEK, α-pERK1/2, α-ERK1/2, α-pAKT, α-AKT, α-pGSK3 α /β, α-GSK3 α /β, α-β catenin, α-pCHK2, α-CHK2, α-tubulin, α-SP1, α-pRET, α-RET (Cell Signaling, Danvers, MA, USA), and α-SOD3 (Santa Cruz, Santa Cruz, CA, USA). Signal density analysis was performed using ImageJ software GEL blot software.
Laukkanen M.O., Cammarota F., Esposito T., Salvatore M, & Castellone M.D. (2015). Extracellular Superoxide Dismutase Regulates the Expression of Small GTPase Regulatory Proteins GEFs, GAPs, and GDI. PLoS ONE, 10(3), e0121441.
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2

Cell Fractionation and Western Blot Analysis

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Cell fractionation was completed essentially as described by the manufacturer (Subcellular Protein Fractionation kit, Pierce; 1×107 human T-ALL cells). However, one additional wash was utilized in each cell fraction. Protein fractions were quantified by Bradford assay and 20μg–45μg of proteins were loaded on a 4–20% gradient gel for Western blot analysis. Primary antibodies were α-TOX, α-TUBULIN (1:500, ab4074, Abcam), α-SP1 (1:1000, #9389, Cell Signaling Technology), and α-H4 (1:1000, #2935, Cell Signaling Technology). The secondary antibodies used were the same as described previously. Cell fractionation was performed three times with similar results.
Lobbardi R., Pinder J., Martinez-Pastor B., Theodorou M., Blackburn J.S., Abraham B.J., Namiki Y., Mansour M., Abdelfattah N.S., Molodtsov A., Alexe G., Toiber D., de Waard M., Jain E., Boukhali M., Lion M., Bhere D., Shah K., Gutierrez A., Stegmaier K., Silverman L.B., Sadreyev R.I., Asara J.M., Oettinger M.A., Haas W., Look A.T., Young R.A., Mostoslavsky R., Dellaire G, & Langenau D.M. (2017). TOX regulates growth, DNA repair, and genomic instability in T-cell Acute Lymphoblastic Leukemia. Cancer discovery, 7(11), 1336-1353.
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