TNT and RDX were provided by HEMRL Pune (India). TNP, 2,4-DNT, 2,6-DNT, and DMNB were purchased from Aldrich, while 1,3-dinitrobenzene (1,3-DNB) and nitrobenzene (NB) were purchased from a local company. All the chemicals were used as received. Dry solvents were used during complete analysis and were obtained locally.
2 4 dnt
Manufactured by Merck Group
Sourced in United States
2,4-DNT is a chemical compound that can be used as a laboratory reagent. It serves as a precursor for various chemical reactions and analyses. However, a detailed description of its core function while maintaining an unbiased and factual approach is not available.
Automatically generated - may contain errors
Lab products found in correlation
2 6 dnt, by Merck Group (4 mentions)
1 3 dnb, by Merck Group (2 mentions)
Hepes buffer, by Merck Group (1 mentions)
Fluoromax 4 spectrofluorometer, by Horiba (1 mentions)
Acetone, by Sinopharm (1 mentions)
Dextrose, by HiMedia (1 mentions)
Isoleucine, by Merck Group (1 mentions)
Histidine, by Merck Group (1 mentions)
Phenylalanine, by Merck Group (1 mentions)
Glutamic acid, by Merck Group (1 mentions)
Maltose, by HiMedia (1 mentions)
Yttrium nitrate hexahydrate, by Merck Group (1 mentions)
Gadolinium nitrate hexahydrate, by Merck Group (1 mentions)
Terbium nitrate hexahydrate, by Merck Group (1 mentions)
Glutamine, by Merck Group (1 mentions)
Aspartic acid, by Merck Group (1 mentions)
Tryptophan, by Merck Group (1 mentions)
2 4 dnp, by Merck Group (1 mentions)
Cysteine, by Merck Group (1 mentions)
Sucrose, by HiMedia (1 mentions)
7 protocols using 2 4 dnt
1
Explosive Compounds Characterization
2
Analysis of Nitroexplosives and Energetic Compounds
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Nitroexplosives, namely, 4-NT,
1,3-DNB, 2,4-DNT, and 2,6-DNT, were purchased from Aldrich Chemicals.
RDX and TNT were purchased from AccuStandard. PA was purchased from
Loba Chemie Pvt. Ltd. HEPES buffer was purchased from Sigma-Aldrich
Chemicals. Various other reagents and chemicals and were purchased
from Merck and Alfa-Aesar and used without further purification. 1H and 13C NMR spectra were recorded at 400 and
100 MHz, respectively, using Varian-AS400 NMR spectrometer. Gel permeable
chromatography (GPC) was performed in CHCl3 using Shimadzu
LC solution GPC instrument with polystyrene as the standard. All of
the experimental titrations were done by using Milli-Q water. PerkinElmer
Lambda-25 spectrophotometer was used to record the UV–vis absorption
spectra. Horiba Fluoromax-4 spectrofluorometer was used to record
the PL spectra by using quartz cuvettes of 10 mm path length and having
a slit width of 1 nm at 298 K.
1,3-DNB, 2,4-DNT, and 2,6-DNT, were purchased from Aldrich Chemicals.
RDX and TNT were purchased from AccuStandard. PA was purchased from
Loba Chemie Pvt. Ltd. HEPES buffer was purchased from Sigma-Aldrich
Chemicals. Various other reagents and chemicals and were purchased
from Merck and Alfa-Aesar and used without further purification. 1H and 13C NMR spectra were recorded at 400 and
100 MHz, respectively, using Varian-AS400 NMR spectrometer. Gel permeable
chromatography (GPC) was performed in CHCl3 using Shimadzu
LC solution GPC instrument with polystyrene as the standard. All of
the experimental titrations were done by using Milli-Q water. PerkinElmer
Lambda-25 spectrophotometer was used to record the UV–vis absorption
spectra. Horiba Fluoromax-4 spectrofluorometer was used to record
the PL spectra by using quartz cuvettes of 10 mm path length and having
a slit width of 1 nm at 298 K.
3
Analytical Grade Chemical Sourcing
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4
2,4-DNT Stock Solution Preparation
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2,4-DNT (101397; ≥97% pure; Sigma-Aldrich, St. Louis, MO, USA) was dissolved in acetone (analytical grade; Sinopharm Chemical Reagent Co., Ltd., Shanghai, China) to prepare a 200 mg/mL 2,4-DNT stock solution. acetone was diluted to obtain a 10 mL/L stock solution. Both stock solutions were stored at 4 °C, away from light.
5
SERS-Based Chemical Detection Using Ag-S Targets
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All the chemicals
and reagents used in our study are of AR grade. Ag target (Ag–S)
of 1 mm thickness (≥99% pure), pristine graphite powder, and
2,4-DNT were purchased from Sigma-Aldrich, and concentrated sulfuric
acid (H2SO4), potassium permanganate (KMnO4), and hydrogen peroxide (H2O2) were
procured from Samir Tech, India. The chemicals and reagents used in
this work were used as received without any further treatment. Deionized
water was used for the SERS sample preparation and cleaning purposes
throughout all the experiments.
and reagents used in our study are of AR grade. Ag target (Ag–S)
of 1 mm thickness (≥99% pure), pristine graphite powder, and
2,4-DNT were purchased from Sigma-Aldrich, and concentrated sulfuric
acid (H2SO4), potassium permanganate (KMnO4), and hydrogen peroxide (H2O2) were
procured from Samir Tech, India. The chemicals and reagents used in
this work were used as received without any further treatment. Deionized
water was used for the SERS sample preparation and cleaning purposes
throughout all the experiments.
6
Synthesis and Characterization of Rare-Earth Doped Nanoparticles
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All the chemicals were of analytical
grade and were used without further purification; yttrium nitrate
hexahydrate (Y(NO3)3·6H2O, 99.89%),
gadolinium nitrate hexahydrate (Gd(NO3)3·6H2O, 99.89%), and terbium nitrate hexahydrate (Tb(NO3)3·6H2O, 99.89%), PEI (with Mw = 25 000 and Mn =
10 000), TNP, 2,4-DNT, 2,6-DNT, 2,4-DNP, RDX, phenylalanine,
cysteine, isoleucine, tryptophan, glutamic acid, aspartic acid, lysine,
histidine, and glutamine were purchased from Sigma-Aldrich Inc. TNT
and RDX were purchased from HEMRL, Pune. NaCl, Na2HPO4, NaH2PO4, Na2CO3, NaHCO3, and NaOH were received from Thomas Baker. NH4F was received from Merck. Dextrose, glucose, sucrose, fructose,
lactose, and maltose were received from Himedia laboratories Pvt.
Ltd. 1,3-DNB, 4-NP, 2,4-DNB, NM, 4-NT, NB, copper oxychloride, cypermethrin,
malathion, fenvelerate, carbendazim, and chloropyrifos were purchased
from a local company and used as received. Ethanol was obtained from
Hayman Ltd. Deionized water was used throughout the experiments.
grade and were used without further purification; yttrium nitrate
hexahydrate (Y(NO3)3·6H2O, 99.89%),
gadolinium nitrate hexahydrate (Gd(NO3)3·6H2O, 99.89%), and terbium nitrate hexahydrate (Tb(NO3)3·6H2O, 99.89%), PEI (with Mw = 25 000 and Mn =
10 000), TNP, 2,4-DNT, 2,6-DNT, 2,4-DNP, RDX, phenylalanine,
cysteine, isoleucine, tryptophan, glutamic acid, aspartic acid, lysine,
histidine, and glutamine were purchased from Sigma-Aldrich Inc. TNT
and RDX were purchased from HEMRL, Pune. NaCl, Na2HPO4, NaH2PO4, Na2CO3, NaHCO3, and NaOH were received from Thomas Baker. NH4F was received from Merck. Dextrose, glucose, sucrose, fructose,
lactose, and maltose were received from Himedia laboratories Pvt.
Ltd. 1,3-DNB, 4-NP, 2,4-DNB, NM, 4-NT, NB, copper oxychloride, cypermethrin,
malathion, fenvelerate, carbendazim, and chloropyrifos were purchased
from a local company and used as received. Ethanol was obtained from
Hayman Ltd. Deionized water was used throughout the experiments.
7
Characterization of Riboswitches with Ligands
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Theophylline, TMR, sodium fluoride, dopamine, thyroxine, 2,4-DNT, DMSO, ascorbic acid and sodium chloride were purchased from Sigma-Aldrich. Luria broth Miller (LB) media was purchased from VWR. All ligand conditions are provided in the Supplementary Data.
DNT has a solubility of 1.48 mM (270 μg/ml) in water. To increase DNT solubility, 1% (v/v) DMSO was added to the media when characterizing DNT riboswitches and no-aptamer controls using the 0 and 1 mM DNT conditions. dopamine is enzymatically oxidized by Escherichia coli DH10B cells, and spontaneously oxidized by molecular oxygen. To minimize dopamine oxidation, 5 mM ascorbic acid was added to cell-free transcription–translation assays to characterize dopamine riboswitches and no-aptamer controls using the 0 and 1 mM dopamine conditions. thyroxine has a solubility of 135 nM (0.105 μg/ml) in neutral water. To increase thyroxine solubility, 10 mM NaOH was added to create a 1 mM thyroxine working stock solution, followed by adding 2 μl of stock solution to 11 μl cell-free transcription–translation assays (154 μM thyroxine). A total of 100 mM NaCl was then added to cell-free transcription–translation assays to maintain physiological RNA folding. Characterization of thyroxine riboswitches and no-aptamer controls took place in the same salt and pH conditions, using either 0 and 154 μM thyroxine.
DNT has a solubility of 1.48 mM (270 μg/ml) in water. To increase DNT solubility, 1% (v/v) DMSO was added to the media when characterizing DNT riboswitches and no-aptamer controls using the 0 and 1 mM DNT conditions. dopamine is enzymatically oxidized by Escherichia coli DH10B cells, and spontaneously oxidized by molecular oxygen. To minimize dopamine oxidation, 5 mM ascorbic acid was added to cell-free transcription–translation assays to characterize dopamine riboswitches and no-aptamer controls using the 0 and 1 mM dopamine conditions. thyroxine has a solubility of 135 nM (0.105 μg/ml) in neutral water. To increase thyroxine solubility, 10 mM NaOH was added to create a 1 mM thyroxine working stock solution, followed by adding 2 μl of stock solution to 11 μl cell-free transcription–translation assays (154 μM thyroxine). A total of 100 mM NaCl was then added to cell-free transcription–translation assays to maintain physiological RNA folding. Characterization of thyroxine riboswitches and no-aptamer controls took place in the same salt and pH conditions, using either 0 and 154 μM thyroxine.
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