Ab181988

Manufactured by Abcam

Sourced in United States

Ab181988 is a laboratory product manufactured by Abcam. It is a tool used for scientific research purposes. No further details can be provided without risk of interpretation or extrapolation.

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Lab products found in correlation

2 protocols using ab181988

Proteomics Analysis of Extracellular Vesicles

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The culture medium was Multicell RPMI 1640, 1× obtained from Wisent Inc.; Saint-Jean-Baptiste, QC, Canada (350-005-CL). Primary antibodies for Western blot analysis were as follows: mouse monoclonal anti-human CD63 (10628D; Invitrogen; Waltham, MA, USA), mouse monoclonal anti-CD81 (10630D; Invitrogen), rabbit monoclonal anti-human glycophorin A (GPA-ab129024; Abcam; Cambridge, UK), rabbit polyclonal anti-P. falciparum Pf GRP78 (BiP, MRA-1246; BEI Resources; Northern Virginia, United States), rabbit monoclonal anti-flotillin 2 (ab181988; Abcam), rabbit monoclonal anti-albumin (ab192603; Abcam), rabbit polyclonal anti-hemoglobin (PA5-97488; Invitrogen). HRP-conjugated secondary antibodies purchased from Abcam were goat anti-mouse IgG (ab6789) and goat anti-rabbit (ab97080). Nalgene 0.45 μm pore PES rapid flow vacuum filter units (165-0045) and Pierce silver stain kit (24612) were from ThermoFisher; Waltham, United States. Satorius vivacell 100 PES concentrators were purchased from Fisher Scientific (VC1042).

Opadokun T., Agyapong J, & Rohrbach P. (2022). Protein Profiling of Malaria-Derived Extracellular Vesicles Reveals Distinct Subtypes. Membranes, 12(4), 397.

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Extracellular Vesicle Protein Profiling

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Proteins (12 μg) from each group were separated by SDS–polyacrylamide gel electrophoresis and then transferred onto polyvinylidene difluoride (PVDF) membranes. PVDF membranes were blocked for 1 hour at room temperature and then incubated with primary antibody overnight at 4°C. The following antibodies were used for Western blot analysis: anti-CD63 (1:1000; Abcam, ab134045), anti-TSG101 (1:1000; Abcam, ab125011), anti-Alix (1:1000; Abcam, ab186429), anti–Flotillin-2 (1:1000; Abcam, ab181988), anti-ARF6 (1:1000; Abcam, ab13126), anti-APOA1 (1:1000; Abcam, ab52945), anti-APOA2 (1:1000; Abcam, ab92478), anti-APOB (1:1000; Abcam, ab139401), and anti-ALB (1:1000; Abcam, ab207327). Horseradish peroxidase–conjugated goat anti-rabbit and goat anti-mouse antibodies were used to detect the bound primary antibodies. The signals were detected by the enhanced chemiluminescence reagent. Data from the bands were determined through ImageJ software.

Wang C., Yang Y., Zhang X., Shi Z., Gao H., Zhong M., Fan Y., Zhang H., Liu B, & Qing G. (2023). Secreted endogenous macrosomes reduce Aβ burden and ameliorate Alzheimer’s disease. Science Advances, 9(21), eade0293.

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