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Galen 3

Manufactured by Leica
Sourced in United States, Germany

The Galen III is a precision laboratory equipment designed for scientific research and analysis. It features advanced optical components and a robust, reliable construction. The Galen III is a versatile tool suitable for a wide range of applications in various fields of study.

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5 protocols using galen 3

1

Spectroscopic Characterization of Compounds

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EIMS were recorded on a Shidmadzu GCMS-QP5050A spectrometer. 1 H NMR and 13 C-NMR spectra were recorded on BRUKER ASCEND TM 700 MHz spectrometer (Karlsruhe, Germany). 1 H NMR and 13 C-NMR spectra were obtained in the above instrument operating at 700 MHz and 175 MHz. Melting points were obtained on a Leica Galen III instrument (Leica Micro-systems, Redwoodcity, CA, USA). Deuterated acetone (acetone-d 6 ) was used in the analysis.
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2

Microscopic analysis of Aleuropleurocelus nymphs

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Fourth instar nymphs of the genus Aleuropleurocelus Drews & Sampson (Hemiptera: Aleyrodidae) were collected from the underside of Karwinskia humboldtiana leaves (Roemer & Schultes) Zucc. (Rhamnaceae), in the Ciudad Victoria Campus at the Autonomous University of Tamaulipas, and on the same plant in the municipality of Llera, Tamaulipas, both in Mexico. Samples were transferred to the Biological Control Laboratory of the Faculty of Engineering and Sciences of the Autonomous University of Tamaulipas, Mexico, where puparia were mounted on slides, and studied under a compound microscope Leica Galen III® (Leica Microsystems, Wetzlar, Germany). Methodology followed for the preparation of specimens was the proposed by Martin (2004) . Preparations were examined in a Leica Galen III microscope at 40×, 100×, 400×, and 1,000×; photographs were taken with a Nikon 5200® (Nikon Corporation, Tokyo, Japan) camera with 18-55 mm lens, directly to the lens eye of the microscope at 100× and 400×.
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3

Histological Analysis of Liver Tissues

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For histological analysis, liver specimens were washed in normal saline and fixed with 10% formalin. Fixed tissues were embedded in paraffin wax, sectioned in rotary microtome (5 μm thick) and then stained with haematoxylin and eosin dyes [31 (link)]. Tissue samples were coded and evaluated for any histological changes using a light microscope (Leica Galen III). and photographed by a digital camera.
Quantitative analysis of the histopathological changes in liver was calculated from an average obtained from observation of six rabbits per group using an ocular micrometer calibrated with a stage micrometer. Evaluating the frequency of the histopathological changes was obtained based on average from observation of 12 microscope fields with an area 625μm2 at 40X or 400X.
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4

Imaging of SA-β-gal-producing Cells

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Images were acquired with a Zeiss LSM780 and a 63× objective as published20 (link) or with a Nikon Optiphot and a 40× objective. Cells producing SA-β-gal were imaged with a Leica Galen III microscope and a 20× objective. For individual experiments, settings were identical for all conditions.
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5

Quantitative and Qualitative Leaf Anatomy

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Quantitative leaf anatomical parameters such as thickness of upper and lower cuticle, thickness of upper and lower epidermis, length of palisade layers all at 40 measurements per parameter (n = 40), as well as qualitative leaf and petiole micro-anatomical characters such as nature of spongy layers, nature of ground tissues, types of trichomes were all observed and documented using light microscope (Leica Galen III). All quantitative parameters were taken with the aid of ocular micrometre. Photomicrographs of the transverse sections of the leaves and petioles were made with the aid of "Accu-scope Trinocular Microscope (Accuscope 33001 LED Trinocular Microscope fixed with 3.2 MP CMOS Digital Camera)."
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