Higuchi-Connors method (1965) was followed to determine the solubility of each polymorphic form[14 ]. An excess amount of each form (100 mg) was added to 25 ml of deionized water in a 100 ml flask with a glass stopper. The flasks were placed on a mechanical shaker and maintained at 37° in thermostatic water bath for 72 h. An aliquot (3 ml) of each solution was withdrawn and filtered through a dialysis membrane 0.45 μ Millipore filter. The amount dissolved was determined by reading the absorbance at 228 nm using a UV/Vis spectrophotometer (Mode l UV-240, Shimadzu, Japan) and using suitably constructed calibration curve.
Model uv 240
Manufactured by Shimadzu
Sourced in Japan
The Shimadzu Model UV-240 is a high-performance ultraviolet-visible (UV-Vis) spectrophotometer. It is designed to measure the absorption or transmission of light in the ultraviolet and visible regions of the electromagnetic spectrum.
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Lab products found in correlation
4 protocols using model uv 240
1
Solubility Determination of Polymorphs
2
Quantifying Vascular Permeability via Evans Blue
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Evans Blue has a high affinity for serum albumin. Increased vascular permeability leads to the extravasation of albumin-bound Evans Blue dye. Evans Blue (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) was injected into the tail vein of each mouse (20 mg/mL in PBS, 100 µg/mouse) and the mouse was left left for 30 min, after which Balb/c mice were anesthetized and perfused with 20–30 mL of PBS. The removed tissue was cut into 5 mm squares. Formamide was added to the minced tissue at a concentration of 0.1 g/mL in order to leak out Evans Blue in the tissues. The absorbance (620 nm) of the leaked Evans Blue was measured using a spectrophotometer (model UV-240, Shimadzu Scientific Instruments, Kyoto, Japan). Dye amounts were calculated from the Evans Blue standard curve (0.5–40 µg/mL).
3
Quantifying Vascular Permeability via Evans Blue
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Evans Blue has a high affinity for serum albumin. Increased vascular permeability leads to the extravasation of albumin-bound Evans Blue dye. Evans Blue (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) was injected into the tail vein of each mouse (20 mg/mL in PBS, 100 µg/mouse) and the mouse was left left for 30 min, after which Balb/c mice were anesthetized and perfused with 20–30 mL of PBS. The removed tissue was cut into 5 mm squares. Formamide was added to the minced tissue at a concentration of 0.1 g/mL in order to leak out Evans Blue in the tissues. The absorbance (620 nm) of the leaked Evans Blue was measured using a spectrophotometer (model UV-240, Shimadzu Scientific Instruments, Kyoto, Japan). Dye amounts were calculated from the Evans Blue standard curve (0.5–40 µg/mL).
4
NMR and Chromatographic Methods for Compound Analysis
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NMR experiments were recorded on Jeol spectrometer (Kyoto, Japan) in DMSO-d6 1H (500 MHz) and 13C (125 MHz) NMR; UV absorption spectra were measured on Shimadzu model-UV-240 (Kyoto, Japan); Column chromatography (CC) was performed using polyamide 6S (Riedel-De-Haen, German) and Sephadex LH-20 (Pharmacia) ; Two dimensional paper chromatography (TDPC) and paper preparative chromatography (PPC) were carried out on Whatman No. 1 and 3 MM paper, respectively, using the following solvent systems: (1) BAW (n-BuOH/AcOH/H2O, 6: O (15:85) .
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