Urea assay kit

Manufactured by Abnova

Sourced in United States, Taiwan, Province of China, Denmark

The Urea Assay Kit is a laboratory equipment that measures the concentration of urea in a sample. It provides a quantitative determination of urea levels without additional interpretation.

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Lab products found in correlation

Spectramax i3 multi mode microplate reader, by Molecular Devices (1 mentions) P450 glo cyp3a4 assay kit, by Promega (1 mentions) Human albumin elisa kit, by Abcam (1 mentions) Fluostar optima plate reader, by BMG Labtech (1 mentions) α gapdh, by Cell Signaling Technology (1 mentions) Pronase, by Merck Group (1 mentions) Collagenase type 1, by Merck Group (1 mentions) Cyquant cell proliferation assay kit, by Thermo Fisher Scientific (1 mentions) α cdk4, by Cell Signaling Technology (1 mentions) αnanog, by Cell Signaling Technology (1 mentions) Dnase 1, by Merck Group (1 mentions) Nicotinamide, by Merck Group (1 mentions) Matrigel, by BD (1 mentions) Hemavet 950fs, by Drew Scientific (1 mentions) Human albumin elisa kit, by Solarbio (1 mentions) Quantichrom creatinine assay kit dict 500, by BioAssay Systems (1 mentions)

8 protocols using urea assay kit

Functional Biomarker Assays of Hepatocytes

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Albumin, urea, and CYP450 enzyme assays were performed as functional biomarkers of the hepatocytes. Human Albumin ELISA Kit (cat# ab108787, Abcam, USA), Urea Assay Kit (cat# KA1652, Abnova, USA), and P450-Glo CYP3A4 Assay Kit (cat# V9001, Promega, USA) were used for albumin, urea, and CYP3A4 quantification, respectively. In brief, cell culture media samples were collected at specified time points and stored at − 80 °C. While CYP3A4 assay was performed by following the manufacturer’s instructions with slight modifications. Media samples were thawed at 37 °C in a water bath before experiments. A microplate reader (SpectraMax i3 Multimode Microplate Reader, Molecular Devices, USA) was used for taking readings by following the manufacturer’s instructions.

Farooqi H.M., Kang B., Khalid M.A., Salih A.R., Hyun K., Park S.H., Huh D, & Choi K.H. (2021). Real-time monitoring of liver fibrosis through embedded sensors in a microphysiological system. Nano Convergence, 8, 3.

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Urea Quantification in Liver Homogenates

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Urea concentration was determined from total liver homogenates. Briefly, samples were prepared from liver homogenates in 0.3 M cold sucrose, which was supplemented with protease inhibitors (Leupeptin at 0.1 mM and PMSF at 0.1 mM), at a 1:4 ratio using 10 up-and-down strokes with a Glass-Teflon homogenizer with a pestle rotation of 1000 rpm. The samples were centrifuged at 1000× g at 4 °C for 15 min, and the supernatant, which was free of nuclei and cellular debris, was used for the determination (Urea Assay Kit, Abnova, Taipei, Taiwan) [13 (link)].

Capiglioni A.M., Capitani M.C., Marrone J, & Marinelli R.A. (2023). Adenoviral Transfer of Human Aquaporin-8 Gene to Mouse Liver Improves Ammonia-Derived Ureagenesis. Cells, 12(11), 1535.

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Urea Concentration Measurement in Biological Fluids

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The urea concentration was measured in both seminal plasma and urine samples with the Urea assay kit (KA 1652, Abnova, Denmark) following the manufacturer’s instructions. The samples, processed with the kits reagents, were placed into a 96-well plate and incubated for 20 min at room temperature. The plate was read in the FLUOstar Optima plate reader (BMG LABTECH) at 520 nm. The readings were transformed and expressed in units of milligrams per deciliter.

González-López W.A., Patel D.M., Duncan N, & Beirão J. (2020). Is it possible to store spotted wolffish (Anarhichas minor) sperm by refrigeration?. Fish Physiology and Biochemistry, 47(3), 725-735.

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Isolation and Culture of Hepatic Progenitor Cells

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Collagenase type I, DNase I, and Pronase were purchased from Sigma Aldrich. The anti-αCD133 antibody was purchased from Miltenyi Biotech. HhAg1.5 was purchased from Cellagen Technology. Cell culture grade dexamethasone and nicotinamide were purchased from Sigma-Aldrich. Recombinant HGF (rHGF), rIL6, rEGF and rFGF2 were purchased from Cell Signaling Technology. 100x Insulin-Tranferrin-Selenium-Ethanolamine (ITS-X ) was purchase from GIBCO.The α-Cyclin D1, α-Cyclin D3, α-CDK4, α-CDK6, α-Nanog and α-GAPDH antibodies were purchased from Cell Signaling Technology. The α-Nanog and α-Sox2 antibodies were purchased from Proteintech and Millipore respectively for western blot purpose. The α-HNF3 and α-TAT antibodies were purchased from R&D systems and Bioworld respectively. The α-FAH was purchased from Sigma Aldrich. For cell sorting, α-CD45 and α-CD133 MACS beads were purchased from Miltenyi Biotec. The anti-αCD133 and its isotype was purchased from ebioscience. Cell proliferation was measured using CyQuant cell proliferation assay kit (Thermo Fisher Scientific). Urea assay kit was purchased from Abnova. Matrigel and Poly-D-Lysine/Laminin coated plates and coverslips were purchased from BD Biosciences.

Mitra A., Yan J., Zhang L, & Li S. (2018). A small molecule Hedgehog agonist HhAg1.5 mediated reprogramming breaks the quiescence of non-injured liver stem cells for rescuing liver failure. Translational research : the journal of laboratory and clinical medicine, 205, 44-50.

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Whole Blood Analysis and Plasma Urea

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For complete blood counts, 10 µL of whole blood was mixed with 10 µL of 2 mM EDTA and counts were performed using the HemaVet 950FS (Drew Scientific). The Urea Assay Kit (Abnova Cat# KA1652) was used to measure plasma urea following the manufacturer's protocol.

Le H.H., Cinaroglu S.S., Manalo E.C., Ors A., Gomes M.M., Duan Sahbaz B., Bonic K., Origel Marmolejo C.A., Quentel A., Plaut J.S., Kawashima T.E., Ozdemir E.S., Malhotra S.V., Ahiska Y., Sezerman U., Bayram Akcapinar G., Saldivar J.C., Timucin E, & Fischer J.M. (2021). Molecular modelling of the FOXO4-TP53 interaction to design senolytic peptides for the elimination of senescent cancer cells. EBioMedicine, 73, 103646.

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Quantifying Albumin and Urea Synthesis

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Human albumin was measured using the Human Albumin ELISA Quantitation kit (Bethyl Laboratory, E80‐129) according to the manufacturer's instructions. Urea synthesis was measured using the Urea Assay Kit (ABNOVA, KA1652) according to the manufacturer's instructions.

Lv Y., Rao Z., Liu L., Jia J., Wu C., Xu J., Du Y., Liu Y., Liu B., Shi J., Li G., Zhao D, & Deng H. (2023). The efficient generation of functional human hepatocytes from chemically induced pluripotent stem cells. Cell Proliferation, 57(2), e13540.

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Quantifying Albumin and Urea Levels

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Human albumin was measured using the Human Albumin ELISA kit (SEKH‐0081, Solarbio) according to the manufacturer's instructions. Urea synthesis was examined with the Urea Assay Kit (KA1652, Abnova) according to the manufacturer's instructions.

Xu Y., Mao S., Fan H., Wan J., Wang L., Zhang M., Zhu S., Yuan J., Lu Y., Wang Z., Yu B., Jiang Z, & Huang Y. (2024). LINC MIR503HG Controls SC‐β Cell Differentiation and Insulin Production by Targeting CDH1 and HES1. Advanced Science, 11(13), 2305631.

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Plasma Electrolytes and Renal Function

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Plasma electrolytes were measured with the CareLyte electrolyte analyzer (Diamond Diagnostic Inc., USA) immediately after collection of blood. Remaining plasma was snap-frozen in liquid nitrogen for further analysis. Plasma creatinine levels were measured using the Quantichrom Creatinine Assay Kit (DICT-500, BioAssay Systems). A standard curve was created from the stock 50 mg/dL creatinine standard (6 mg/dL, 2 mg/dL, 1 mg/dL, 0.5 mg/dL and 0 mg/dL). Creatinine concentrations were determined by measuring absorbance per the manufacturer’s instructions. BUN was measured using a urea assay kit (Abnova, #KA1652) according to manufacturers’ instructions.

Polina I., Spicer M.J., Domondon M., Schibalski R.S., Sarsenova E., Sultanova R.F, & Ilatovskaya D.V. (2021). Inhibition of neprilysin with sacubitril without RAS blockage aggravates renal disease in Dahl SS rats. Renal Failure, 43(1), 315-324.

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