Dulbecco’s Modified Eagle’s Medium (DMEM), phosphate-buffered saline (PBS), trypsin, monodansylcadaverine (MDC) stain, propidium iodide (PI), ribonuclease A (RNase A) and all other additional chemicals not specifically mentioned were of analytical grade and were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Penicillin, streptomycin and fungizone were bought from Thermo Fisher Scientific (Waltham, MA, USA), and fetal calf serum (FCS) was purchased from Biochrom GmbH (Berlin, Germany). Crystal violet, 1% glutaraldehyde and Triton X-100 were purchased from EMD Millipore (Billerica, MA, USA). Ethics approval was obtained from The Research Ethics Committee, Faculty of Health Sciences, University of Pretoria, South Africa (reference number: 258/2015).
Monodansylcadaverine mdc stain
Manufactured by Merck Group
Sourced in United States, Germany
Monodansylcadaverine (MDC) is a fluorescent dye used in cell biology research. It serves as a selective stain for autophagic vacuoles, allowing the visualization and quantification of autophagic activity in cells.
Automatically generated - may contain errors
Lab products found in correlation
Triton x 100, by Merck Group (1 mentions)
Crystal violet, by Merck Group (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
Trypsin, by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Fungizone, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Merck Group (1 mentions)
Fetal calf serum (fcs), by Harvard Bioscience (1 mentions)
Ribonuclease a rnase a, by Merck Group (1 mentions)
2 protocols using monodansylcadaverine mdc stain
1
Apoptosis Assay in Cell Lines
2
Quantifying Autophagic Vacuoles via MDC Staining
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The autophagic vacuoles were quantified in cells after the PS-PDT treatment using the monodansylcadaverine (MDC) stain (Sigma Aldrich, Germany). Two hours post-irradiation, the cells were stained with 0.05 mM MDC in PBS for 15 min at 37°C, washed with PBS, followed by fluorescence microscopy (excitation/emission wavelengths: 335/498 nm).
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