TMC was quantified using GTK-M agar plates (MILCOM a.s., Tábor, Czech Republic) by culturing at 30 °C for 72 h under the aerobic conditions, according to ČSN EN ISO 4833-1 [14 ], which is the Czech equivalent of ISO 4833-1.
Identification of bacteria and their count: pathogenic bacteria in milk were determined by the Veterinary Laboratory Vedia s.r.o. (Strakonice, Czech Republic). The presence of coliform bacteria, staphylococci, streptococci, and corynebacteria was monitored. Basic cultivation of bacteria occurred on the Columbia blood agar (Oxoid, UK). ENDO agar (Oxoid, UK) was used to cultivate coliform bacteria. Enterococcus Selective Agar-BAA (Oxoid, UK) was used to distinguish streptococci and enterococci, and Edwards agar (Oxoid, UK) was used for streptococcal culture, and for staphylococci, Baird-Parker agar (Oxoid, UK) and Staphylococcus agar (Sigma-Aldrich, USA) was applied. A coagulase test was used to distinguish between coagulase-negative and -positive staphylococci (Staphylase Test, Oxoid, UK).
When necessary, a microflex LT MALDI TOF mass spectrometer (Bruker Daltonics, Bremen, Germany) with a microSCOUT ion source and a TOF flight time analyzer (Bruker Daltonics, Bremen, Germany) in conjunction with the MALDI Biotyper software system (Bruker Daltonics, Bremen, Germany) was used to identify bacterial species.
Identification of bacteria and their count: pathogenic bacteria in milk were determined by the Veterinary Laboratory Vedia s.r.o. (Strakonice, Czech Republic). The presence of coliform bacteria, staphylococci, streptococci, and corynebacteria was monitored. Basic cultivation of bacteria occurred on the Columbia blood agar (Oxoid, UK). ENDO agar (Oxoid, UK) was used to cultivate coliform bacteria. Enterococcus Selective Agar-BAA (Oxoid, UK) was used to distinguish streptococci and enterococci, and Edwards agar (Oxoid, UK) was used for streptococcal culture, and for staphylococci, Baird-Parker agar (Oxoid, UK) and Staphylococcus agar (Sigma-Aldrich, USA) was applied. A coagulase test was used to distinguish between coagulase-negative and -positive staphylococci (Staphylase Test, Oxoid, UK).
When necessary, a microflex LT MALDI TOF mass spectrometer (Bruker Daltonics, Bremen, Germany) with a microSCOUT ion source and a TOF flight time analyzer (Bruker Daltonics, Bremen, Germany) in conjunction with the MALDI Biotyper software system (Bruker Daltonics, Bremen, Germany) was used to identify bacterial species.