100 nM of RFC:PCNA was applied on carbon-coated 400-mesh grids. Excess sample was blotted from the grid surface, the grids were washed twice with 50 mM HEPES, pH 7.5 and stained with 1% uranyl acetate. RFC:PCNA was imaged on a 120 kV Philips CM-120 microscope fitted with a Gatan Orius SC1000 detector.
Orius sc1000 detector
Manufactured by Ametek
The Orius SC1000 is a high-performance digital camera detector designed for electron microscopy applications. It features a large, high-resolution sensor and advanced electronics to capture high-quality images with excellent detail and contrast.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using orius sc1000 detector
1
Visualization of RFC:PCNA Complex
2
Thermus thermophilus Virus Imaging
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Infections of Thermus thermophilus HB8 were performed as described above (growth/purification of P74-26 virions). Aliquots of the infection culture were diluted 1:1 in Thermus growth medium and applied to carbon-coated 400-mesh grids 1 h after infection. Excess sample was then blotted from the grid surface, and grids were finally stained with 1% uranyl acetate. Data were then collected on a 120 kV Philips CM-120 microscope fitted with a Gatan Orius SC1000 detector.
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