Dual luciferase reporter gene assay kit rg027

p16sheLL plasmid encoding HPV16 L1 and L2 (Plasmid #37320, Addgene) was kindly donated by Dr. John Schiller (NIH, Bethesda USA); pRL-SV40-C (D2768), pNFκB-TA-Luc (D2207), pAP1-TA-Luc plasmids (D2109) and Dual Luciferase Reporter Gene Assay Kit (RG027) were purchased from Beyotime Biotechnology, Shanghai, China.

Human promyelocytic leukemia cells (HL-60) and acute lymphatic leukemia cells (CCRF-CEM) were obtained from the Shanghai Cell Bank of the Chinese Academy of Sciences. DAC (A119533) was purchased from Aladdin (China). Lipofectamine® 2000 (11668-027) was purchased from Invitrogen (China). Opti-MEM (M5650) was purchased from Sigma (USA). The dual luciferase reporter gene assay kit (RG027) was purchased from Beyotime (China). The cell counting kit 8 (CCK8) (C1706) was purchased from Bioswamp (China). The SYBR FAST qPCR Master Mix (KM4101) was purchased from KAPA Biosystems (USA). The oligo DT18/miR-135a-5p RT Primer (3806), recombinant RNase inhibitor (2313A), and PrimeScript II RTase (2690A) were purchased from TAKARA (Japan). BCA protein concentration assay kit (PC0020) was purchased from Solarbio (China), DAB (DA1010) was purchased from Solarbio (China), and all antibodies (anti-Bad PAB32756; anti-Bcl-2 PAB30599; anti-cleaved-caspase-3 MAB37300; anti-GAPDH PAB36269; goat anti-rabbit IgG SAB43714) were purchased from Bioswamp (China).

Anti-Flag (F1804-5mg), anti-Myc (05-419), and anti-hemagglutinin (HA; H9658) mouse monoclonal antibodies (MAbs) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-Myc (R1208-1) and anti-Flag (0912-1) rabbit polyclonal antibodies (PAbs), anti-lamin B1 (ET1606-27) rabbit MAb, and anti-β-actin (EM21002) mouse MAb were purchased from HuaAn Biotechnology (Hangzhou, China). Anti-TRAF3 (D260776-0100) rabbit PAb for Western blotting (WB) was purchased from Sangon Biotech (Shanghai, China). Mouse MAbs against IBDV proteins (VP1, VP2, VP3, VP4, and VP5) were prepared in our laboratory (59 (link)). Anti-IRF3 (11312-1-AP) rabbit PAb was purchased from Proteintech (Wuhan, China). Phospho-IRF3 (Ser386) (number 4947) and phospho-TBK1 (number 5483) rabbit MAbs were purchased from Cell Signaling Technology (Danvers, MA, USA). A dual-luciferase reporter gene assay kit (RG027) was purchased from Beyotime (Shanghai, China). Exfect transfection reagent (T101-01/02) for plasmids was purchased from Vazyme Biotechnology (Nanjing, China). jetPRIME transfection reagent (PT-114-15; Polyplus, New York, NY, USA) was used for RNAi transfection. A human IFN-β ELISA kit (JL19215) was purchased from Jianglai Biotechnology (Shanghai, China). Puromycin (ant-pr-1), poly(I·C) (tlrl-pic), and VACV-70 (tlrl-vav70n) were purchased from InvivoGen (San Diego, CA).