Human civ

Manufactured by Merck Group

Sourced in United States

Human CIV is a laboratory equipment product designed for research purposes. It serves as a platform for the cultivation and study of human cells. The core function of this product is to provide a controlled and standardized environment for cell culture experiments.

Automatically generated - may contain errors

Lab products found in correlation

Bovine serum albumin (bsa), by Merck Group (1 mentions) Microplate reader uv max, by Beckman Coulter (1 mentions) Ap114p, by Merck Group (1 mentions) Ap113p, by Merck Group (1 mentions)

3 protocols using human civ

ELISA for Serum Antibodies to Coronavirus

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serum antibodies (IgG, IgM and IgA) to CIV were measured by an enzyme-linked immunosorbent assay (ELISA). In brief, each well of the microtiter plate was sensitized with 100 µl of 10 µg/ml of human CIV (SIGMA, USA) at room temperature for 3 h, followed by an overnight incubation at 4°C.

The plate was washed with phosphate-buffered saline (PBS) containing 0.05% Tween 20 and 1% bovine serum albumin (BSA, SIGMA, USA).

Then, 100 µl serum sample (diluted 1: 10), was placed in each well of a microtiter plate, and incubated for 1 h at 37°C.

After washing three times, 100 µl of immunoconjugates (anti-human immunoglobulin peroxidase conjugates (SIGMA, USA) to heavy chain of IgG, IgM and IgA) were added to each well for 1 h at 37°C. All immunoconjugates were diluted 1: 10,000 with PBS containing 1% BSA and 0.05% Tween 20.

The plate was incubated for 1 h at 37°C. o-Phenylenediamine (0.4 mg/ml) was added to citrate buffer, and 100 µl of this solution was added to each well and allowed to react for 30 min.

The reaction was stopped by adding 50 µl 4 M H₂SO₄ to each well and the optical density was measured with a Microelisa Reader 210 (Organon Teknika, Belgium) at a wavelength of 492 nm.

Nikolov A., Tsinlikov I., Tsinlikova I., Nicoloff G., Blazhev A, & Garev A. (2016). Serum anti-collagen type IV IgM antibodies and development of diabetic nephropathy in diabetics with essential hypertension. Central-European Journal of Immunology, 41(1), 86-92.

+ Open protocol

+ Expand

Sandwich ELISA for Measuring IgM Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols

To measure ACIVAbs IgM concentrations, a sandwich ELISA was used. The assay was performed as follows: a microtiter 96-well polystyrene plate was coated with 100 μL of 10 μg/mL of human CIV (Sigma-Aldrich, St. Louis, MO, USA) at room temperature for 3 h, followed by overnight incubation at 4 °C. The plate was washed with phosphate-buffered saline (PBS) containing 0.05% Tween 20 and 1% bovine serum albumin (BSA; Cat. No. A2153, Sigma-Aldrich, St. Louis, MO, USA). Then, a 100-μL serum sample (diluted 1:10) was placed in each well of a microtiter plate and incubated for 1 h at 37 °C. After washing three times, 100 μL of goat anti-human IgM Ab, Fc5µ, HRP conjugate (AP114P, Sigma-Aldrich, St. Louis, MO, USA) were added to each well for 1 h at 37 °C. All immunoconjugates were diluted 1:10,000 with PBS containing 1% BSA and 0.05% Tween 20. The plate was incubated for 1 h at 37 °C. Ortho-phenylenediamine (4 mg/mL in 0.05 M citrate buffer, pH 5.0 with H₂O₂) was used as a colorimetric substrate. The reaction was stopped by adding 50 μL of 4 M H₂SO₄ to each well, and the optical density was measured with a micro-ELISA plate reader (Coulter Microplate Reader UV Max) at a wavelength of 492 nm.

Kostov K, & Blazhev A. (2020). Use of Glycated Hemoglobin (A1c) as a Biomarker for Vascular Risk in Type 2 Diabetes: Its Relationship with Matrix Metalloproteinases-2, -9 and the Metabolism of Collagen IV and Elastin. Medicina, 56(5), 231.

+ Open protocol

+ Expand

ELISA for CIV-specific IgM and IgG

Check if the same lab product or an alternative is used in the 5 most similar protocols

To measure the ACIVAbs IgM and ACIVAbs IgG concentrations, a sandwich ELISA was used. The assays were performed as follows: microtiter 96-well polystyrene plates were coated with 100 μL of 10 μg/mL of human CIV (Sigma-Aldrich, St. Louis, MO, USA), followed by an overnight incubation at 4 °C. Then, 100 μL serum sample (diluted 1:10) was placed in each well of the microtiter plates and incubated for 1 h at 37 °C. After washing, 100 μL of goat anti-human IgM Ab, Fc5µ, HRP conjugate (AP114P, Sigma-Aldrich, St. Louis, MO, USA), or goat anti-human IgG Ab, Fc, and HRP conjugate (AP113P, Sigma-Aldrich, St. Louis, MO, USA), respectively, were added to each well for 1 h at 37 °C. All immunoconjugates were diluted 1:10,000. Then, 100 μL of ortho-phenylenediamine (4 mg/mL in 0.05 M citrate buffer) was added as a colorimetric substrate for 30 min. The reaction was stopped by adding 50 μL of 4 M H₂SO₄ to each well, and the optical density was measured with a micro-ELISA plate reader (Coulter Microplate Reader UV Max, Molecular Devices Corp., Menlo Park, CA, USA) at a wavelength of 492 nm.

Kostov K, & Blazhev A. (2021). Serum Anti-Collagen IV IgM and IgG Antibodies as Indicators of Low Vascular Turnover of Collagen IV in Patients with Long-Term Complications of Type 2 Diabetes. Diagnostics, 11(5), 900.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!