N succinimidyl 3 2 pyridyldithio propionate

Manufactured by Merck Group

Sourced in Spain

N-succinimidyl 3-(2-pyridyldithio)propionate is a heterobifunctional crosslinking reagent used for coupling biomolecules. It contains an N-hydroxysuccinimide ester group for reaction with primary amines and a pyridyldithio group for reaction with sulfhydryl groups.

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Lab products found in correlation

C reactive protein (crp), by Merck Group (1 mentions) Alexa fluor 647 nhs, by Thermo Fisher Scientific (1 mentions)

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Propionate (76 citations)

2 protocols using n succinimidyl 3 2 pyridyldithio propionate

Preparation and Purification of CD105-Targeted Immunotoxin

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Common chemicals and biological materials were purchased from the same sources as reported elsewhere [12 (link)]. WST-1 reagent was from Roche Diagnostics, S.L. Life Science (Sant Cugat del Vallés, Spain) N-succinimidyl 3-(2-pyridyldithio)propionate was obtained from Sigma Chemical Co (St. Louis, MO, USA). Saporin S5 was a generous gift from Prof. F. Stirpe (Università di Bologna, Bologna, Italy). The hybridoma cell line 44G4 producing a monoclonal antibody (mAb) to hCD105 was kindly provided by Dr. Michelle Letarte through Dr. C. Bernabéu (Centro de Investigaciones Biologicas, CSIC, Madrid, Spain). It was grown as described elsewhere: Purification of 44G4 mAb was performed by protein A-Sepharose chromatography as described previously [21 (link)]. Mouse fibroblasts L929-shCD105+ expressing the short human form of CD105 were a generous gift from Dr. C. Bernabéu [35 (link)].

Barriuso B., Antolín P., Arias F.J., Girotti A., Jiménez P., Cordoba-Diaz M., Cordoba-Diaz D, & Girbés T. (2016). Anti-Human Endoglin (hCD105) Immunotoxin—Containing Recombinant Single Chain Ribosome-Inactivating Protein Musarmin 1. Toxins, 8(6), 184.

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Fluorescent Labeling of GSAO and CRP

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GSAO and control compound 4‐(N‐(S‐glutathionylacetyl)amino)benzoic acid were synthesized previously as described²⁵ and conjugated to amine‐reactive succinimidyl ester Alexa Fluor 647‐NHS (Life Technologies). Confirmation of GSAO activity and batch to batch validation testing after conjugation were performed as previously described.¹⁷, ²⁶ Monomeric collagen‐related peptide (CRP, Auspep) was cross‐linked using N‐succinimidyl 3‐(2‐pyridyldithio)propionate (Sigma‐Aldrich) to produce CRP‐xL. Reagents are available on request.

Lee C.S., Selvadurai M.V., Pasalic L., Yeung J., Konda M., Kershaw G.W., Favaloro E.J, & Chen V.M. (2022). Measurement of procoagulant platelets provides mechanistic insight and diagnostic potential in heparin‐induced thrombocytopenia. Journal of Thrombosis and Haemostasis, 20(4), 975-988.

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