Lung tissue dissociation kit

Manufactured by Miltenyi Biotec

Sourced in Germany

The Lung Tissue Dissociation Kit is a laboratory product designed to isolate and prepare single-cell suspensions from lung tissue samples. The kit contains the necessary enzymes, buffers, and reagents to effectively dissociate the lung tissue into individual cells, which can then be used for further analysis or experiments.

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Spelling variants of this product

Lung Dissociation Kit (78 citations) Tissue dissociation kit (30 citations)

2 protocols using lung tissue dissociation kit

Dissociation and Analysis of Murine Tumor Immune Cells

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Mice were perfused with DMEM to remove circulating blood. Lung and tumors were dissected and washed in DMEM. Tissue was minced and enzymatically digested following manufacture protocol (Miltenyi lung tissue dissociation kit, 130–095-927). Single-cell suspensions were then lysed with RBC lysis buffer (ThermoFisher 00–4333-57). Final single-cell suspension was stained with CD45-APCY7 (Biolegend, 103116), GR1-PB (Biolegend, 108430), CD11b-PE (BD, 552850), CD-19-FITC (Biolegend, 152404), CD3-APC (Biolegend, 100236), and Dapi(live/dead) on ice and then analyzed on BD FORTESSA. Cells were gated on DAPI negative, CD45+ and further defined as monocytes (Gr^loCD11b⁺, CD3⁻CD19⁻), granulocytes (Gr^hiCD11b⁺, CD3⁻CD19⁻), T cells (CD3⁺) and B cells (CD19⁺).

Hilfenhaus G., Mompeón A., Freshman J., Prajapati D.P., Hernandez G., Freitas V.M., Ma F., Langenbacher A.D., Mirkov S., Song D., Cho B.K., Goo Y.A., Pellegrini M., Chen J.N., Damoiseaux R, & Iruela-Arispe M.L. (2020). A high-content screen identifies drugs that restrict tumor cell extravasation across the endothelial barrier. Cancer research, 81(3), 619-633.

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Protein Extraction from Murine Lungs

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To extract proteins from the lung tissues of mice, isolated right lungs were homogenized using a lung tissue dissociation kit (130-095-927, Miltenyi Biotec, Bargisch Gladbach, Germany). After homogenization, the samples were collected in DPBS and were lysed in ice-cold cell lysis buffer with a protease inhibitor. To separate the cytoplasm and nucleus, the NE-PER™ Nuclear and Cytoplasmic Extraction Reagent (Thermo Fisher Scientific) was used. The concentration of extracted proteins in each sample was measured using the Bradford protein assay. The expression of IκBα and β-actin in the cytoplasmic fraction and NF-κB p65 and lamin B1 in the nuclear fraction were measured using the ProteinSimple Wes capillary immunoassay system (Protein Simple, San Jose, CA, USA).

Kim S.Y., Shin D.U., Eom J.E., Jung S.Y., Song H.J., Lim K.M., Kim G.D., Yun S.I., Kim M.Y., Shin H.S, & Lee S.Y. (2022). Artemisia gmelinii Attenuates Lung Inflammation by Suppressing the NF-κB/MAPK Pathway. Antioxidants, 11(3), 568.

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