Si circhipk3

Specific small interfering RNA (siRNA) against circHIPK3 (si-circHIPK3) and siRNA scrambled control (si-NC), and short hairpin RNA objecting circHIPK3 (sh-circHIPK3) and shRNA scrambled control (sh-NC) were purchased from GenePharma (Shanghai, China). The miR-876-5p mimic (miR-876-5p) and its negative control (miR-NC), and miR-876-5p inhibitor (anti-miR-876-5p) and its negative control (anti-miR-NC) were designed and acquired from Thermo Fisher Scientific (Waltham, MA, USA). HGC-27 and AGS cells were transfected with plasmids or oligonucleotides using Lipofecamine2000 (Invitrogen, Carlsbad, CA, USA). The sequences for si-circHIPK3, si-NC, sh-circHIPK3 and sh-NC were as follows: si-circHIPK3: 5′-CUACAGGUAUGGCCUCACA-3′, si-NC: 5′-UUCUCCGAACGUGUCACGUTT-3′, sh-circHIPK3: 5′-ccggCUACAGGUAUGGCCUCACAttcaagagaTGTGAGGCCATACCTGTAGTTTTTTGGTACC-3′, sh-NC: 5′- ccggUUCUCCGAACGUGUCACGUTTttcaagagaAATCGTGACACGTTCGGAGAATTTTTTGGTACC-3′. For overexpression of PIK3R1 (PIK3R1), the primers were used for amplification and then cloned in the mammalian expression pcDNA3.1 vector (Invitrogen), PIK3R1 F-5′-CCGGAATTCATGAGTGCTGAGGGGTACCAGTAC-3′; and R-5′-CCGCTCGAGATCGCCTCTGCTGTGCATATATA-3′.