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Ab109906

Manufactured by Abcam
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Ab109906 is a recombinant antibody that specifically recognizes human EGFR (epidermal growth factor receptor). The antibody is produced in HEK293 cells and is purified using protein A affinity chromatography.

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Lab products found in correlation

Ab109903, by Abcam (3 mentions) Ab109907, by Abcam (3 mentions) Ab109905, by Abcam (2 mentions) Xf96 extracellular flux analyzer, by Agilent Technologies (2 mentions) Citrate synthase activity assay kit, by Abcam (1 mentions)

3 protocols using ab109906

1

Metformin Effects on Hepatocyte Mitochondria

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Primary hepatocytes were seeded in an XF 96 well plate coated with 0.01% collagen type 1.36 h after seeding, cells were washed with PBS twice, and glucose production medium was added with different concentrations of metformin for 6 h. Mitochondrial respiratory chain activity was determined by using Seahorse XF96 Extracellular Flux Analyzers in Seahorse assay medium (0.55 mg/ml pyruvate in base medium, pH7.4). After determination of basal oxygen consumption rates, cells were sequentially treated with oligomycin A (1 μM), FCCP(1 μM), and rotenone (1 μM) along with antimycin A(1 μM). Viable cell numbers were counted and used to normalize the oxygen consumption rate.
Different metformin concentrations were used to determine the effects of metformin on mitochondrial activity of complex I (ab109903, abcam), complex II+III (ab109905, abcam), complex IV (ab109906, abcam), and complex V (ab109907, abcam) in in vitro assays following the manufacturer’s procedures.
Wang Y., An H., Liu T., Qin C., Sesaki H., Guo S., Radovick S., Hussain M., Maheshwari A., Wondisford F.E., O’Rourke B, & He L. (2019). Metformin Improves Mitochondrial Respiratory Activity through Activation of AMPK. Cell reports, 29(6), 1511-1523.e5.
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2

Mitochondrial Respiratory Complex Analysis

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Cells were washed twice with phosphate-buffered saline and incubated for 30 min on ice in lysis buffer (68 mM sucrose, 200 mM mannitol, 50 mM potassium chloride, 1 mM ethylenediaminetetraacetic acid (EDTA), 1 mM EGTA, and 1 mM dithiothreitol with a protease inhibitor cocktail. The cells were then lysed using 45 passages through a 25G 5/8 needle and centrifuged at 1,500 g for 10 min. Cytosolic extracts were recovered after centrifugation at 13,000 g for 20 min. The pellet contained the mitochondria. The total protein concentration was determined using the BCA kit (Pierce, USA) according to the manufacturer’s instructions. The mitochondrial activity of complex I (ab109903, Abcam), complex II + III (b109905, Abcam), complex IV (ab109906, Abcam), and complex V (ab109907, Abcam) was determined using in vitro assays following the manufacturer’s procedures. The citrate synthase activity of mitochondrial extracts was measured using a Citrate Synthase Activity Assay Kit (ab119692, Abcam) according to the manufacturer’s instructions.
Ma Y., Chen G., Yi J., Li Q., Tan Z., Fan W., Luo X., He Z., Si Z, & Li J. (2022). IRX3 plays an important role in the pathogenesis of metabolic-associated fatty liver disease by regulating hepatic lipid metabolism. Frontiers in Endocrinology, 13, 895593.
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3

Metformin Effects on Hepatocyte Mitochondria

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary hepatocytes were seeded in an XF 96 well plate coated with 0.01% collagen type 1.36 h after seeding, cells were washed with PBS twice, and glucose production medium was added with different concentrations of metformin for 6 h. Mitochondrial respiratory chain activity was determined by using Seahorse XF96 Extracellular Flux Analyzers in Seahorse assay medium (0.55 mg/ml pyruvate in base medium, pH7.4). After determination of basal oxygen consumption rates, cells were sequentially treated with oligomycin A (1 μM), FCCP(1 μM), and rotenone (1 μM) along with antimycin A(1 μM). Viable cell numbers were counted and used to normalize the oxygen consumption rate.
Different metformin concentrations were used to determine the effects of metformin on mitochondrial activity of complex I (ab109903, abcam), complex II+III (ab109905, abcam), complex IV (ab109906, abcam), and complex V (ab109907, abcam) in in vitro assays following the manufacturer’s procedures.
Wang Y., An H., Liu T., Qin C., Sesaki H., Guo S., Radovick S., Hussain M., Maheshwari A., Wondisford F.E., O’Rourke B, & He L. (2019). Metformin Improves Mitochondrial Respiratory Activity through Activation of AMPK. Cell reports, 29(6), 1511-1523.e5.
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