Mdck mdr1 cell lines

The nine drugs tested (amitryptiline, atenolol, carbamazepine, fleroxacin, loperamide, norfloxacin, pefloxacin, propranolol and zolpidem) and HPLC grade solvents (acetonitrile, methanol and water) were purchased from Sigma-Aldrich (Barcelona, Spain). MDCK cell line was purchased from ATCC (USA) and MDCK-MDR1 cells were provided by Dr. Gottessman, MM (Nathional Institutes of Health, Bethesda). Pig brain homogenate was kindly supplied by a local slaughterhouse and fresh unfertilized chicken eggs were bought in a local supermarket. Table 2 shows the molecular properties of the nine drugs mentioned above.
Dulbecco’s modified Eagle’s medium (DMEM) with high content of glucose, L-glutamine, HEPES, MEM non-essential aminoacid, penicillin−streptomycin, trypsin-EDTA, Hank’s balanced salt solution (HBSS) and fetal bovine serum (FBS) for the cell culture of MDCK and MDCK-MDR1 cell lines were purchased from Sigma-Aldrich.

The six drugs used for constructing the mathematical model (amitriptyline, caffeine, carbamazepine, fleroxacin, pefloxacin and zolpidem) and HPLC-grade solvents (acetonitrile, methanol and water) were purchased from Sigma-Aldrich (Barcelona, Spain). The MDCK cell line was purchased from ATCC (USA), MDCK-MDR1 cells were provided by Dr. Gottessman, and the MM (National Institutes of Health, Bethesda, MD, USA) and hCMEC/D3 cell lines were purchased from Cedarlane (Burlington, ON, Canada). Pig brain homogenate was kindly supplied by a local slaughterhouse.
Dulbecco’s modified eagle’s medium (DMEM) with a high content of glucose, L-glutamine, HEPES, MEM non-Essential aminoacid, penicillin−streptomycin, trypsin-EDTA, Hank’s balanced salt solution (HBSS) and fetal bovine serum (FBS) for the cell culture of MDCK and MDCK-MDR1 cell lines were purchased from Sigma-Aldrich (Barcelona, Spain).
The products needed for the culture of hCMEC/D3 cells were purchased from Sigma-Aldrich (Barcelona, Spain) (hydrocortisone, ascorbic acid, HEPES, Triton X-100 and bFGF), Gibco (Barcelona, Spain) (FBS, penicillin–streptomycin, chemically defined lipid concentrate, HBSS, collagen I rat protein and trypsin-EDTA), Lona (Barcelona, Spain) (EBM-2 medium).