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5 protocols using orthophosphoric acid hplc grade 85

1

Antioxidant Potential of Protein Matrices

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Almond protein matrix was purchased from Raab Vitalfood GmbH (Rohrbach, Germany) and brown rice protein matrix was from Kernnel premium (Zagreb, Croatia). Quercetin, trolox and 2,2-diphenyl-1-picrylhydrazyl (DPPH) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Sodium carbonate was obtained from Kemika (Zagreb, Croatia). Neocuproine, copper (II) chloride and 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) were obtained from Acros Organics (Geel, Belgium). Orthophosphoric acid (HPLC grade > 85%) was obtained from Fisher Scientific (Loughborough, UK), while methanol (HPLC grade) was purchased from J.T. Baker (Deventer, The Netherlands). Iron (III) chloride hexahydrate, ethanol, sodium acetate and ammonium acetate were purchased from Gram-mol (Zagreb, Croatia).
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2

Antioxidant and Anti-Diabetic Potential of Pectin and Apple Fiber

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The apple fiber powder was supplied by Biesterfeld AG (Zagreb, Croatia) and both types of pectin were the product of CP Kelco (Atlanta, GA, USA). The 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), trolox, 4-(dimethylamino)-cinnamaldehyde (DMAC), chlorogenic, caffeic, p-coumaric, gallic and ellagic acids, rutin, quercetin, phloretin and phlorizin were purchased from Sigma-Aldrich (St. Louis, MO, USA). The α-glucosidase (from Saccharomyces cerevisiae) and acarbose were obtained from Sigma-Aldrich (St. Louis, MO, USA). The 4-nitrophenyl-α-D-glucopyranoside was obtained from Alfa Aesar (Kandel, Germany) and potassium dihydrogen phosphate was from BDH Prolabo (Poole, UK). Potassium persulfate, Folin-Chiocalteu reagent and sodium carbonate were obtained from Kemika (Zagreb, Croatia). Neocuproine, 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) and copper (II) chloride were purchased from Acros Organics (Geel, Belgium). Methanol (HPLC grade) was from J.T. Baker (Deventer, The Netherlands) and orthophosphoric acid (HPLC grade > 85%) was from Fisher Scientific (Loughborough, UK). Iron (III) chloride hexahydrate, sodium acetate, ethanol, ammonium acetate and starch were bought from Gram-mol (Zagreb, Croatia). Cyanidin 3-glucoside was from Extrasynthese (Genay, France).
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3

Comprehensive Phytochemical Analysis of Apple Fibers

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Apple fibers were obtained from Biesterfeld AG (Zagreb, Croatia). 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 4-(dimethylamino)-cinnamaldehyde (DMAC), trolox, 2,2-diphenyl-1-picrylhydrazyl (DPPH), rutin, quercetin, phloretin, phlorizin, chlorogenic, caffeic, p-coumaric, gallic, ellagic acids, myrtenol and α-amylase (from the porcine pancreas) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Potassium persulfate, Folin–Ciocalteu reagent and sodium carbonate were purchased from Kemika (Zagreb, Croatia). Neocuproine, 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) and copper (II) chloride were bought from Acros Organics (Geel, Belgium). Methanol (HPLC grade) was from J.T. Baker (Deventer, Netherlands) and orthophosphoric acid (HPLC grade > 85%) was from Fisher Scientific (Loughborough, UK). Iron (III) chloride hexahydrate, sodium acetate, ethanol, ammonium acetate and starch were bought from Gram-mol (Zagreb, Croatia). Cyanidin 3-glucoside was from Extrasynthese (Genay, France) and 3,5-dinitrosalicylic acid from Alfa Aesar (Kandel, Germany).
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4

Citrus Fiber Antioxidant Potential Analysis

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Citrus fiber (76.1% of dietary fiber, while the rest are proteins—7%, sugars—7.2%, fats—0.77%, minerals—3.06%, and moisture—5.85%) was obtained from Fiberstar (River Falls, WI, USA). Potassium persulfate and sodium carbonate were purchased from Kemika (Zagreb, Croatia). Neocuproine, hesperidin, 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) and copper (II) chloride were bought from Acros Organics (Geel, Belgium). Trolox, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 4-(dimethylamino)-cinnamaldehyde (DMAC), quercetin, caffeic acid, p-coumaric acid, chlorogenic acid, rutin, gallic acid and ellagic acid were obtained from Sigma-Aldrich (St. Louis, MO, USA). Cyanidin 3-glucoside was from Extrasynthese (Genay, France). Methanol (HPLC grade) was from J.T. Baker (Deventer, Netherlands), and orthophosphoric acid (HPLC grade > 85%) was from Fisher Scientific (Loughborough, UK). Iron (III) chloride hexahydrate, sodium acetate, ethanol, ammonium acetate and Folin–Ciocalteu reagent were bought from Gram-mol (Zagreb, Croatia).
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5

Analytical Characterization of Natural Compounds

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CMC was purchased from CP Kelco (Äänekoski, Finland). Sucrose was a product of Gram-mol (Zagreb, Croatia). Trehalose was obtained from Hayashibara doo (Nagase group, Tokyo, Japan). Hydrochloric acid was from Carlo Erba Reagents (Sabadell, Spain). Sodium carbonate was from T.T.T. (Sveta Nedelja, Croatia). Folin–Ciocalteu reagent and potassium persulfate were from Kemika (Zagreb, Croatia). Trolox, 2,2-diphenyl-1-picrylhydrazil, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, and most of the standards used (rutin, quercetin, quercetin-3-D-glucoside, chlorogenic acid) were products of Sigma-Aldrich (St. Louis, MO, USA), while the standards of cyanidin-3-glucoside, cyanidin-3-galactoside, cyanidin-3-arabinoside, neochlorogenic acid, and quercetin-3-D-galactoside were products of Extrasynthese (Genay, France). Cupric chloride, neocuproine, and 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) were purchased from Acros Organic (Geel, Belgium). Methanol (HPLC grade) was from J.T. Baker (Deventer, Netherlands) and orthophosphoric acid (HPLC grade, >85%) was from Fisher Scientific (Longhborough, UK).
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