Anti eea1 610457

Manufactured by BD

The Anti-EEA1 610457 is a laboratory reagent used for the detection and localization of the Early Endosome Antigen 1 (EEA1) protein in biological samples. EEA1 is a marker for early endosomes, which are important organelles involved in the endocytic pathway. This antibody can be used in various applications, such as immunohistochemistry, immunocytochemistry, and Western blotting, to study the distribution and expression of EEA1 in cells and tissues.

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Lab products found in correlation

Gar igg h l po antibodies, by Thermo Fisher Scientific (1 mentions) 2 5 dihydroxybenzoic acid, by Merck Group (1 mentions) Hrp conjugated donkey anti mouse igg 715 035 150, by Jackson ImmunoResearch (1 mentions) Hrp conjugated anti mouse igg 711 035 152, by Jackson ImmunoResearch (1 mentions) Gefitinib sml1657, by Merck Group (1 mentions) Anti ubiquitin sc 8017, by Santa Cruz Biotechnology (1 mentions) Anti rhogdi sc 360, by Santa Cruz Biotechnology (1 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Dq red bsa d12051, by Thermo Fisher Scientific (1 mentions) Anti hsp90 sc 13119, by Santa Cruz Biotechnology (1 mentions)

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Anti-EEA1 (36 citations)

2 protocols using anti eea1 610457

Immunostaining Protocols for HSV Antigens

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Rabbit anti-HSV glycoprotein B and D (gB/gD) antibody was kindly provided by E. Tabares. Antibodies that recognized viral proteins gC and ICP4 were supplied by Abcam [anti-HSV1 gC Envelope Protein (3G9) and anti-HSV 1 ICP4 Immediate Early Protein [10F1]). Mouse monoclonal anti-tubulin (clone B-5-1-2; T5168), anti-SAMHD1 Abs (SAB1101454 and HPA047072) were obtained from Sigma; and anti-EEA1 610457 from BD Biosciences. Anti-CD81 5A6 monoclonal antibody was kindly donated by S. Levy (Standford).
The secondary antibodies used for immunostaining were horseradish peroxidase-coupled anti-mouse (Vector; PI-2000) and anti-rabbit (Nordic; GAR/IgG(H + L)/PO) antibodies, and species-matching secondary antibodies coupled to 488 and 647 Alexa Fluor fluorochromes (ThermoFisher).

Benayas B., Sastre I., López-Martín S., Oo A., Kim B., Bullido M.J., Aldudo J, & Yáñez-Mó M. (2020). Tetraspanin CD81 regulates HSV-1 infection. Medical Microbiology and Immunology, 209(4), 489-498.

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Quantifying EGF-mediated cellular signaling

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EGF (10ng/ml) was purchased from PeproTech. Anti-UBTD1 (HPA034825), sodium chloride, DAPI, Gefitinib (SML1657) and 2,5-dihydroxybenzoic acid (DHB) were purchased from Sigma-Aldrich. Anti-HSP90 (sc-13119), anti-RhoGDI (sc-360) and anti-Ubiquitin (sc-8017) were purchased from Santa Cruz Biotechnology. Anti-pSTAT3 (clone D3A7, 9145), anti-STAT3 (clone 124H6, 9139), anti-p-EGF receptor (2234) and anti-EGF receptor (2232) were purchased from Cell Signaling. Anti-EEA1 (610457) and anti-CD107a/LAMP1 (555798) were purchased from BD Biosciences. Anti-SQSTM1/P62 (GTX100685) was purchased from Genetex. Anti-calreticulin-ER Marker (ab2907) was purchased from Abcam. DQ™ Red BSA (D12051) was purchased from Invitrogen. Ganglioside GM3 (860058) was purchased from Avanti Polar Lipids, HRP-conjugated donkey anti-mouse IgG (715-035-150) and HRP-conjugated anti-mouse IgG (711-035-152) were purchased from Jackson ImmunoResearch Laboratories. Epidermal Growth Factor, biotinylated, complexed to Alexa Fluor 647 Streptavidin (Alexa Fluor 647 EGF complex) was purchased from Molecular Probes, Invitrogen (E35351).

Torrino S., Tiroille V., Dolfi B., Dufies M., Hinault C., Bonesso L., Dagnino S., Uhler J.P., Irondelle M., Gay A., Fleuriot L., Debayle D., Lacas‐Gervais S., Cormont M., Bertero T., Bost F., Gilleron J., & Clavel S. (2020). UBTD1 regulates ceramide balance and endolysosomal positioning to coordinate EGFR signaling.

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