Reagents were purchased from
Sigma-Aldrich or Tokyo Chemical Industry (TCI) and used as received.
Methylfluorescein (1 ), HSN2, DNS-N3, and C7-Az,
and AP39 were synthesized as reported previously.31 ,40 (link),65 ,66 (link),71 (link) Deuterated solvents were purchased from Cambridge
Isotope Laboratories and used as received. Silica gel (SiliaFlash
F60, Silicycle, 230–400 mesh) was used for column chromatography.
Preparatory chromatography was performed on Silicycle SiliaPlates
(1 mm thickness). 1H and 13C{1H}
NMR spectra were recorded on a Varian INOVA 500 MHz NMR instrument.
Chemical shifts are reported in ppm relative to residual protic solvent
resonances. UV–visible spectra were acquired on a Cary 100
spectrometer equipped with a Quantum Northwest TLC-42 dual cuvette
temperature controller at 37.00 ± 0.05 °C. Fluorescence
spectra were obtained on a Quanta Master 40 spectrofluorometer (Photon
Technology International) equipped with a Quantum Northwest TLC-50
temperature controller at 37.0 ± 0.05 °C.
Sigma-Aldrich or Tokyo Chemical Industry (TCI) and used as received.
Methylfluorescein (
and AP39 were synthesized as reported previously.31 ,40 (link),65 ,66 (link),71 (link) Deuterated solvents were purchased from Cambridge
Isotope Laboratories and used as received. Silica gel (SiliaFlash
F60, Silicycle, 230–400 mesh) was used for column chromatography.
Preparatory chromatography was performed on Silicycle SiliaPlates
(1 mm thickness). 1H and 13C{1H}
NMR spectra were recorded on a Varian INOVA 500 MHz NMR instrument.
Chemical shifts are reported in ppm relative to residual protic solvent
resonances. UV–visible spectra were acquired on a Cary 100
spectrometer equipped with a Quantum Northwest TLC-42 dual cuvette
temperature controller at 37.00 ± 0.05 °C. Fluorescence
spectra were obtained on a Quanta Master 40 spectrofluorometer (Photon
Technology International) equipped with a Quantum Northwest TLC-50
temperature controller at 37.0 ± 0.05 °C.