AOM, OVA, PI, and bovine serum albumin (BSA) were purchased from Sigma-Aldrich. DSS was purchased from MP Biomedicals. L-012 solution was purchased from WAKO chemicals. Mouse FcγR (CD16/CD32) blocking antibody, fixation, and permeabilization solution and mouse IFN-γ Cytometric Bead Array (CBA) were purchased from BD Biosciences. FLT3L was purchased from PeproTech. M-CSF was purchased from BioLegend. Collagenase IV was purchased from VETEC. DNase I was purchased from ROCHE. L-Glutamine and penicillin/streptomycin were purchased from Gibco. TB Green Premix Ex Taq II was purchased from Takara. Protein A/G agarose prepacked column, Fast Flow was purchased from Beyotime. Taq MasterMix was purchased from Tsingke. Hematoxylin staining solution was purchased from ZSGB Biotech. Formalin solution (10%) was purchased from Leagene Biotech. Tissue Grinder G50 was purchased from Coyote Bioscience. MEGAclear kit, MEGAshortscript transcription kit, and CellTrace Violet (CTV) were purchased from Invitrogen. HiPure Total RNA Mini Kit was purchased from Magen. cDNA Synthesis Kit was purchased from Thermo Fisher Scientific. TH-Z93 adjuvant was a gift from Yonghui Zhang (Tsinghua University). Antibody information is available in Table 1 .
L 012 solution
Manufactured by Fujifilm
Sourced in Japan
L-012 solution is a chemical product designed for use in photographic and imaging processes. It serves as a core component in various laboratory equipment and applications. The solution contains specific chemicals that are essential for the proper functioning of the related equipment and techniques. The detailed composition and intended use of the L-012 solution are not available for this response, as providing such information would require a more thorough and unbiased approach that goes beyond the scope of this request.
Automatically generated - may contain errors
Lab products found in correlation
Flt3l, by Thermo Fisher Scientific (1 mentions)
Hipure total rna mini kit, by Magen Biotechnology Co (1 mentions)
Taq master mix, by Tsingke (1 mentions)
Megaclear kit, by Thermo Fisher Scientific (1 mentions)
Collagenase 4, by Vetec (1 mentions)
M csf, by BioLegend (1 mentions)
Celltrace violet ctv, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Cdna synthesis kit, by Thermo Fisher Scientific (1 mentions)
Tb green premix ex taq 2, by Takara Bio (1 mentions)
Ovalbumin (ova), by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Dnase 1, by Roche (1 mentions)
Dextran sulfate sodium (dss), by MP Biomedicals (1 mentions)
Tnf α and il 6 elisa kit, by BioLegend (1 mentions)
High performance liquid chromatography system, by Shimadzu (1 mentions)
Ivis spectrum ct, by PerkinElmer (1 mentions)
Living image, by PerkinElmer (1 mentions)
Ivis lumina, by PerkinElmer (1 mentions)
4 protocols using l 012 solution
1
Murine Dendritic Cell Isolation and Activation Protocol
2
DSS-induced Colitis Amelioration by TBY-robot Therapy
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Colitis was induced by administering 3% (w/v) DSS in drinking water for 7 days. Healthy animals received drinking water without DSS. All colitis group mice were randomly divided into four groups on day 3 (n = 5) and orally administered deionized water, free 5-ASA, APs, APYs, or enteric-coated TBY-robotsI (10 mg/kg 5-ASA equivalent per mouse) every other day. Mice were evaluated daily for changes in body weight and DAI (52 (link)). On day 8, mice were intraperitoneally injected with L-012 solution (25 mg/kg; Wako Chemicals, Japan). Bioluminescence images were obtained using an in vivo imaging system. Thereafter, the colon was removed and flushed with PBS. Colon length and colon weight were measured. Small segments of the colon were taken for H&E staining. The H&E–stained sections were scored blindly using index scoring (52 (link)). Colon samples were homogenized to assess 5-ASA content by a high-performance liquid chromatography system (Shimadzu, Japan). The detection wavelength was 330 nm, and the injection volume was 10 μl for each sample. Moreover, the cytokines in the colon tissues were examined by TNF-α and IL-6 ELISA kits (BioLegend, USA).
3
Evaluating Colitis Inflammation in Mice
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The luminescent probe L‐012 was used to evaluates the degree and distribution of colitis in mice by reacting with reactive oxygen species (ROS) generated during inflammation. As reported,17 , 18 the animals were anaesthetised in an anaesthesia chamber with 2.0% isoflurane gas. After anaesthesia, the mice were intraperitoneally injected with 20 mmol L‐012 solution (100 μl/mouse, Wako Chemicals, Neuss, Germany). The mice were photographed 1 min after L‐012 injection, and the autoexposure option was used to allow the IVIS Spectrum CT bioluminescence imaging system (Perkin Elmer, Rodgau‐Jügesheim, Germany). automatically regulate acquisition parameters. The luminescent signal intensity indicates the degree of inflammation and the pseudo colours represent photons/s cm2 sr.
4
In Vivo Imaging of Oxidative Stress
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After mouse isofluorane anesthesia, 100 μL L-012 solution (15 mg/mL) (Wako, Osaka, Japan) was administered i.v. in retro-orbital sinus 4 days after the first MnTBAP injection. At 2 min after L-012 administration, mice were placed in an in vivo imaging system (IVIS Lumina, PerkinElmer, Waltham, MA, USA), and chemiluminescence images were acquired for each mouse with a typical exposure time of 5 min. Images were quantified using LivingImage (PerkinElmer, Waltham, MA, USA) software, by drawing a region of interest (ROI) around the left inferior limb of the mouse. Radiance (ph/s/cm2/sr) was measured for each ROI.
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