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Goat anti mouse iga

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Goat anti-mouse IgA is a secondary antibody produced in goats that binds to mouse immunoglobulin A (IgA) antibodies. It can be used to detect and quantify mouse IgA in various immunoassays and research applications.

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Lab products found in correlation

Microplate reader, by Thermo Fisher Scientific (2 mentions) Electroblotting, by Bio-Rad (1 mentions) Enhanced chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) Protein g resin, by GenScript (1 mentions) Goat anti mouse igg, by Jackson ImmunoResearch (1 mentions) Mini protean tgx gel, by Bio-Rad (1 mentions) Goat anti mouse igm, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

Mouse anti-goat (5 citations) Goat anti-mouse IgA antibody (2 citations)

2 protocols using goat anti mouse iga

1

Quantifying anti-rOmpA antibodies by ELISA

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The levels of anti-rOmpA antibodies in serum, BALF, and other mucosal washes samples were determined by enzyme-linked immunosorbent assay (ELISA). Briefly, 96-well plates were coated with rOmpA at a concentration of 0.5μg/mL overnight at 4°C and blocked with 3% BSA in PBS for 2 h at 37°C. The serum samples were added to wells at a dilution of 1:10000, while oral, intestinal, urethral washes and BALF samples were added to wells at a dilution of 1:2. HRP-conjugated goat anti-mouse IgG, IgG1, IG2a (diluted 1:8000) (Santa Cruz) and goat anti-mouse IgA (diluted 1:8000) (Santa Cruz) were used as the secondary antibody. Color was developed by adding 100μL 3, 3’, 5, 5’- tetramethylbenzidine (TMB) (Solarbio, China). The plates were kept at 37°C for 15 min and the color development was stopped by adding 100μL 1mmol/ mL H2SO4 in each well. The absorbance of the reaction system was measured at 450 nm by using a microplate reader (Thermo Fisher).
Li Y., Tang X., Zhao Z., Wang H., Wang X., Shang X., Liu P., Kou Z., Jiang Y, & Li Y. (2019). Intranasal immunization with recombinant outer membrane protein A induces protective immune response against Stenotrophomonas maltophilia infection. PLoS ONE, 14(4), e0214596.
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2

Immunoprecipitation and Immunoblotting of MLP

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109 CFU of WT E. coli (K-12) were lysed in 1 mL of lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% (v/v) NP-40, and 1 mM PMSF [pH 7.5]) and sonicated. 200 μL of cell-free supernatant was first incubated with 20 μL of Protein G resin (50% slurry) (GenScript) at 4° C for 1 hr, and supernatant was then incubated with anti-MLP (5 μg/mL) overnight at 4° C before incubation with 20 μL of Protein G resin at 4° C for 2 hr. After that, Protein G resin was pelleted, washed five times with cold lysis buffer, and resuspended in 30 μL of lysis buffer for immunoblotting. Proteins were separated by 4%–20% precast gels (Mini-PROTEAN TGX Gels; Bio-Rad) and transferred to PVDF membranes by electroblotting (BioRad), and membranes were immunoblotted with WT mouse serum (1: 200), human serum (1:400), or anti-mouse monoclonal MLP (1:2,000) overnight at 4° C. Proteins were detected with goat anti-mouse IgG, goat anti-mouse IgM, goat anti-human IgG (Jackson ImmunoResearch Laboratories), or goat anti-mouse IgA (Santa Cruz Biotechnology) and enhanced chemiluminescent substrate (Thermo Scientific).
Zeng M.Y., Cisalpino D., Varadarajan S., Hellman J., Warren H.S., Cascalho M., Inohara N, & Núñez G. (2016). Gut Microbiota-Induced Immunoglobulin G Controls Systemic Infection by Symbiotic Bacteria and Pathogens. Immunity, 44(3), 647-658.
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