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Cd45ro pe vio770

Manufactured by Miltenyi Biotec
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CD45RO-PE-Vio770 is a fluorochrome-conjugated antibody that recognizes the CD45RO isoform of the CD45 antigen. CD45 is a transmembrane protein tyrosine phosphatase that is expressed on all nucleated hematopoietic cells. The CD45RO isoform is expressed on memory T cells.

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Lab products found in correlation

Macsquant analyzer flow cytometer, by Miltenyi Biotec (3 mentions) Cd27 vioblue, by Miltenyi Biotec (3 mentions) Cd3 percp, by Miltenyi Biotec (3 mentions) Cd8 fitc, by Miltenyi Biotec (3 mentions) Cd38 apc, by Miltenyi Biotec (3 mentions) Cd4 apc vio770, by Miltenyi Biotec (3 mentions) Hla dr pe, by Miltenyi Biotec (2 mentions) Cd8 viogreen, by Miltenyi Biotec (2 mentions) Cd45 v450, by BD (1 mentions) 7 aad, by Merck Group (1 mentions) Viobility 405 520 fixable dye, by Miltenyi Biotec (1 mentions) Gallios flow cytometer, by Beckman Coulter (1 mentions) Cd34 pe, by Miltenyi Biotec (1 mentions) Cd3 apc vio770, by Miltenyi Biotec (1 mentions) Celltrace violet, by Thermo Fisher Scientific (1 mentions) Cd4 apc, by Miltenyi Biotec (1 mentions) Cd20 pe vio770, by Miltenyi Biotec (1 mentions) Cd62l vioblue, by Miltenyi Biotec (1 mentions) Cd56 pe, by Miltenyi Biotec (1 mentions) Cd3 pe, by Miltenyi Biotec (1 mentions)

Close spelling variants of this product

CD45RO-PE

5 protocols using cd45ro pe vio770

1

Immunophenotyping of Peripheral Blood

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Phenotypes and activation markers were evaluated by Miltenyi Biotec flow cytometer-MACSQuant Analyzer (8 fluorescence channels, three lasers) on freshly isolated peripheral blood mononuclear cells. Immune activation was evaluated by multiparameter flow cytofluorimetric analysis by the following anti-human monoclonal antibodies: CD3-PerCP, CD4-APC-Vio770, CD8-FITC, CD45RO-PE-Vio770, CD27-VioBlue, CD38-APC, and HLA-DR-PE (Miltenyi Biotec, Bergisch Gladbach, Germany).
Trinchieri V., Laghi L., Vitali B., Parolin C., Giusti I., Capobianco D., Mastromarino P, & De Simone C. (2017). Efficacy and Safety of a Multistrain Probiotic Formulation Depends from Manufacturing. Frontiers in Immunology, 8, 1474.
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2

Flow Cytometric Analysis of CD8+ T cells

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Flow cytometric analysis of experiments was performed on a Gallios Flow Cytometer (Beckmann Coulter, Brea, CA, USA). Antibodies used for CD8+ T cm enrichment were: CD62L-phycoerythrin (PE) (clone: 145/15; Miltenyi, Bergisch-Gladbach, Germany), CD45RO-PE-Vio770 (clone: UCHL1; Miltenyi), and propidium iodide (PI) (Thermo Fisher Scientific, Waltham, MA, USA). For flow cytometric analysis the following antibodies were used: CD3-PE-Vio615 (clone: REA613; Miltenyi), CD8-peridinin chlorophyll protein complex (PerCP)-Vio700 (clone: BW 135/80; Miltenyi), CD8-VioGreen (clone: BW 135/80; Miltenyi), and CD45-V450 (clone: HI30) (BD Biosciences). Live/dead cell discrimination was performed with 7-AAD (Sigma-Aldrich, St. Louis, MO, USA) and Viobility 405/520 Fixable Dye (Miltenyi). Cell doublets were excluded from analysis.
Melzer M.K., Zeitlinger L., Mall S., Steiger K., Schmid R.M., Ebert O., Krackhardt A, & Altomonte J. (2018). Enhanced Safety and Efficacy of Oncolytic VSV Therapy by Combination with T Cell Receptor Transgenic T Cells as Carriers. Molecular Therapy Oncolytics, 12, 26-40.
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3

Flow Cytometry Immunophenotyping Panel

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MACSQuant Analyzer, MACSQuant Analyzer VYB (Miltenyi Biotec), or FACSCanto, LSRII (Becton Dickinson, NJ) was used to analyze cell populations by flow cytometry. The following antibody and protein conjugates were used: CD3-PE, CD3-APC-Vio770, CD4-APC, CD4-VioGreen, CD8-APC-Vio770, CD8-VioGreen, CD14-APC, CD16-PE, CD19-APC, CD20-PerCP-Vio770, CD20-PE-Vio770, CD34-APC, CD34-PE, CD45-VioBlue, CD45-VioGreen, CD45RO-PE-Vio770, CD56-PE, CD62L-VioBlue, CD95-APC, CD45-VioBlue (mouse) (all from Miltenyi Biotec); ErbB2-Fc fusion protein (R&D Systems), anti-human-IgG (Fc gamma-specific) PE (eBioscience). 7AAD staining was used for dead cell exclusion and Violet Cell Trace (Thermo Fisher) for SupT1 cell tracking.
Radek C., Bernadin O., Drechsel K., Cordes N., Pfeifer R., Sträßer P., Mormin M., Gutierrez-Guerrero A., Cosset F.L., Kaiser A.D., Schaser T., Galy A., Verhoeyen E, & Johnston I.C. (2019). Vectofusin-1 Improves Transduction of Primary Human Cells with Diverse Retroviral and Lentiviral Pseudotypes, Enabling Robust, Automated Closed-System Manufacturing. Human Gene Therapy, 30(12), 1477-1493.
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4

Flow Cytometry Analysis of PBMCs

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PBMC were collected and aliquoted in 1 × 106 cells/mL RPMI medium plus 10% Fetal Bovine Serum and then washed by centrifugation. The following anti-human monoclonal antibodies were added: CD3-PerCP, CD4+-APC-Vio770, CD8+-FITC, CD4+ 5RO-PEVio770, CD27-VioBlue, CD38-APC, (Miltenyi Biotec, Bergisch Gladbach, Germany). Samples were acquired by Miltenyi Biotec flow cytometer-MACSQuant Analyzer (8 fluorescence channels, 3 lasers). Gating and data analysis were performed using MACSQuantify software 2.5 (Miltenyi Biotec, Bergisch Gladbach, Germany).
Innocenti G.P., Santinelli L., Laghi L., Borrazzo C., Pinacchio C., Fratino M., Celani L., Cavallari E.N., Scagnolari C., Frasca F., Antonelli G., Mastroianni C.M., d’Ettorre G, & Ceccarelli G. (2020). Modulation of Phenylalanine and Tyrosine Metabolism in HIV-1 Infected Patients with Neurocognitive Impairment: Results from a Clinical Trial. Metabolites, 10(7), 274.
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5

Multiparameter Flow Cytometric Analysis

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Phenotypes and activation markers were evaluated by Miltenyi Biotec flow cytometer-MACSQuant Analyzer (8 fluorescence channels, 3 lasers) on freshly isolated PBMC. Immune activation was evaluated by multiparameter flow cytofluorimetric analysis by the following antihuman monoclonal antibodies: CD3-PerCP, CD4-APC-Vio770, CD8-FITC, CD45ROPE-Vio770, CD27-VioBlue, CD38-APC, and HLA-DR-PE (Miltenyi Biotec, Bergisch Gladbach, Germany).
Scheri G.C., Fard S.N., Schietroma I., Mastrangelo A., Pinacchio C., Giustini N., Serafino S., De Girolamo G., Cavallari E.N., Statzu M., Laghi L., Vullo A., Ceccarelli G., Vullo V, & d’Ettorre G. (2017). Modulation of Tryptophan/Serotonin Pathway by Probiotic Supplementation in Human Immunodeficiency Virus–Positive Patients: Preliminary Results of a New Study Approach. International Journal of Tryptophan Research : IJTR, 10, 1178646917710668.
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