Alexa flour 647 antibody labeling kit

Manufactured by Thermo Fisher Scientific

The Alexa Fluor 647 antibody labeling kit is a tool designed to enable the conjugation of the Alexa Fluor 647 fluorescent dye to antibodies or other proteins. The kit provides the necessary components, including the reactive dye, to facilitate the labeling process. This allows for the detection and visualization of the labeled proteins using fluorescence-based techniques.

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Lab products found in correlation

Protein g sepharose 4 fast flow, by GE Healthcare (1 mentions)

Close spelling variants of this product

Alexa Flour 647 Alexa Flours Antibody labeling kit Alexa 647

2 protocols using alexa flour 647 antibody labeling kit

Thrombosis and Hemostasis Assay

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Slounase, batroxobin and activated factor X were obtained from Lee’s
Pharmaceutical Holdings Limited (Hong Kong China). Unfractionated
Heparin was acquired from SAGENT Pharmaceuticals (IL, USA). DyLight
488 anti-GPIbβ was obtained from Emfret Analytics (Eibelstadt,
Germany). Anti-mouse fibrin antibody was a kind gift from Dr. R.
Camire (Children’s Hospital of Philadelphia) and was fluorescently
labeled using an Alexa Flour 647 antibody labeling kit (Thermo
Fisher).

Adili R., Jackson M., Stanger L., Dai X., Li M., Li B.X, & Holinstat M. (2021). Slounase, a Batroxobin Containing Activated Factor X Effectively Enhances Hemostatic Clot Formation and Reducing Bleeding in Hypocoagulant Conditions in Mice. Clinical and Applied Thrombosis/Hemostasis, 27, 10760296211018510.

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Lupus IgG Binding and Uptake in Neutrophils

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IgG from a lupus patient (SLE1) was purified with Protein G Sepharose 4 Fast flow (GE Healthcare). The purified IgG were labeled with Alexa Flour 647 Antibody Labeling Kit (ThermoFisher Scientific). The Alexa-647 labeled IgG were mixed with Sm/RNP to generate RNA-containing ICs. Neutrophils were treated with anti-FcγRIIA Ab, anti-FcγRIII Ab or chloroquine for 15 min and the ICs were added to the cells in RPMI1640 supplemented with 10% FBS and penicillin-streptomycin. After a 1-hour incubation at 4°C and 37°C, the cells were washed twice with ice-cold PBS supplemented with 3% FBS and 2mM EDTA. The cells were fixed with 2% formaldehyde in PBS. The binding and/uptake of Alexa647-labeled ICs were analyzed with flow cytometry.

Bonegio R.G., Lin J.D., Beaudette-Zlatanova B., York M.R., Menn-Josephy H, & Yasuda K. (2019). Lupus-associated immune complexes activate human neutrophils in an FcγRIIA-dependent but TLR-independent response. Journal of immunology (Baltimore, Md. : 1950), 202(3), 675-683.

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