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Viia7 real time instrument

Manufactured by Thermo Fisher Scientific
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The ViiA7 real-time instrument is a high-performance real-time PCR system designed for accurate and reliable nucleic acid analysis. It features a 96-well format and supports a wide range of applications, including gene expression analysis, genotyping, and copy number variation studies.

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Lab products found in correlation

Viia7 ruo software version 1, by Thermo Fisher Scientific (2 mentions) Taqman technology, by Thermo Fisher Scientific (1 mentions) Taqman universal master mix, by Thermo Fisher Scientific (1 mentions) Microrna rt kit, by Thermo Fisher Scientific (1 mentions) Ambion cells to ct kit, by Thermo Fisher Scientific (1 mentions)

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ViiA7 (579 citations) ViiA7 instrument (129 citations)

4 protocols using viia7 real time instrument

1

SNP Genotyping Using TaqMan and KASP

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The SNP genotyping was conducted in the Genomic Epidemiology laboratory at the German Cancer Research Center (DKFZ), Heidelberg using TaqMan (ABI, Applied Biosystems, Foster City, CA) and KASP (KBioscence, Hoddesdon, UK) Technologies and TaqMan Genotyping Master Mix (Applied Bioscience) technology, according to the manufacturers’ instructions. All samples were included in a 384-well plate. For quality control, duplicates of 5% of the samples were included. Polymerase chain reaction plates were read on a ViiA7 real time instrument (Applied Biosystems). The ViiA7 RUO Software, version 1.2.2 (Applied Biosystems), was used to determine genotypes. The genotyping concordance between duplicate samples exceeded 99%, and samples with a call rate lower than 75% were discarded from the statistical analysis. rs35226131 was monomorphic in our population, therefore it was not included in further analyses.
Aoki M.N., Stein A., de Oliveira J.C., Chammas R., Uno M., Munhoz F.B., Marin A.M, & Canzian F. (2021). Susceptibility loci for pancreatic cancer in the Brazilian population. BMC Medical Genomics, 14, 111.
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2

Genotyping DNA Samples from PANDoRA Consortium

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The sample preparation and genotyping process were conducted at a single laboratory at German Cancer Research Center in Heidelberg, Germany. DNA extraction from the whole blood of both cases and controls within the PANDoRA consortium was carried out using a Qiagen-manufactured kit (Hilden, Germany). To ensure uniformity, the order of DNA samples from case and control subjects was randomized on plates, guaranteeing an equal representation of cases and controls in each batch. Genotyping was conducted via allele-specific PCR-based TaqMan technology (ThermoFisher, Applied Biosystems, Waltham, MA) by ordering TaqMan SNP Genotyping Assays for the selected seven SNPs. The PCR protocol was performed with TaqMan Genotyping Master Mix in 384-well plates following the manufacturer's recommendations. The PCR plates were read on a ViiA7 real-time instrument (Applied Biosystems), and genotypes were determined using the ViiA7 RUO Software, version 1.2.2 (Applied Biosystems).
Ünal P., Lu Y., Bueno-de-Mesquita B., van Eijck C.H., Talar-Wojnarowska R., Szentesi A., Gazouli M., Kreivenaite E., Tavano F., Małecka-Wojciesko E., Erőss B., Oliverius M., Bunduc S., Nóbrega Aoki M., Vodickova L., Boggi U., Giaccherini M., Kondrackiene J., Chammas R., Palmieri O., Theodoropoulos G.E., Bijlsma M.F., Basso D., Mohelnikova-Duchonova B., Soucek P., Izbicki J.R., Kiudelis V., Vanella G., Arcidiacono P.G., Włodarczyk B., Hackert T., Schöttker B., Uzunoglu F.G., Bambi F., Goetz M., Hlavac V., Brenner H., Perri F., Carrara S., Landi S., Hegyi P., Dijk F., Maiello E., Capretti G., Testoni S.G., Petrone M.C., Stocker H., Ermini S., Archibugi L., Gentiluomo M., Cavestro G.M., Pezzilli R., Di Franco G., Milanetto A.C., Sperti C., Neoptolemos J.P., Morelli L., Vokacova K., Pasquali C., Lawlor R.T., Bazzocchi F., Kupcinskas J., Capurso G., Campa D, & Canzian F. (2024). Polymorphisms in transcription factor binding sites and enhancer regions and pancreatic ductal adenocarcinoma risk. Human Genomics, 18, 12.
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3

Validated miRNA Expression Analysis

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miRNA validation was carried out using TaqMan real-time quantitative PCR (qPCR). Reverse transcription (RT) was performed using miRNA-specific stem-loop RT primers and a MicroRNA RT Kit (Life Technologies) following the manufacturer’s instructions. The resulting cDNA was diluted and used immediately for qPCR or stored at −20°C until use. miRNA expression levels were measured by real-time qPCR in a ViiA 7 Real-Time instrument (Life Technologies) using TaqMan® Universal Master Mix (Life Technologies). The miRNA expression levels were normalized to those of cel-miR-39 and determined by the equation 2–ΔCt, where ΔCt=cycle threshold (Ct) (miRNA) – Ct (cel-miR-39).
Hu H., Zhao M., Li Z., Nie H., He J., Chen Z., Yuan J., Guo H., Zhang X., Yang H., Wu T, & He M. (2022). Plasma miR-193b-3p Is Elevated in Type 2 Diabetes and Could Impair Glucose Metabolism. Frontiers in Endocrinology, 13, 814347.
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4

Quantifying Cardiac Marker Expression in iPSC-CMs

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RNA from iPS cell derived cardiomyocytes was isolated using the Ambion Cells-to-Ct Kit (Life Technologies) according to the manufacturer. Cardiomyocytes were dissociated as described above. To quantify the expression of pan-cardiac marker cardiac troponin T (cTnT) and ventricular specific marker myosin light chain 2 (MYL2) during cardiac differentiation, quantitative polymerase chain reaction (QPCR) was performed using pre-designed TaqMan assays (S1 Table). QPCR reagents were assembled in 384-well plates using 10 μL Taqman Gene Expression Mastermix, 5 μL of nuclease free water, 1 μL of the Taqman Gene Expression Assay of choice and 4 μL of cDNA. The PCRs were run on a ViiA7 real time instrument (Life Technologies) using the following program: 2 minutes at 50°C, 10 minutes at 95°C, 40 cycles of 95°C for 15 seconds and 1 minute at 60°C. Relative gene expression was calculated with the ΔΔCt method normalizing target gene expression to B2M [24 (link)].
Fuerstenau-Sharp M., Zimmermann M.E., Stark K., Jentsch N., Klingenstein M., Drzymalski M., Wagner S., Maier L.S., Hehr U., Baessler A., Fischer M, & Hengstenberg C. (2015). Generation of Highly Purified Human Cardiomyocytes from Peripheral Blood Mononuclear Cell-Derived Induced Pluripotent Stem Cells. PLoS ONE, 10(5), e0126596.
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