Striatal TNF-α, NF-κB p65, and caspase-3 were estimated using rat ELISA kits (RayBiotech, Inc., Norcross, GA). Moreover, striatal IL-6 and BDNF were estimated using rat ELISA kits purchased from R and D system (Minneapolis, MN, USA) and MyBioSource Inc. (San Diego, CA, USA), respectively. The procedures were performed according to the manufacturer’s instructions. The results are expressed as pg/mg protein for TNF-α, IL-6, BDNF, and NF-κB p65 and ng/mg protein for caspase-3.
Rat elisa kit
Manufactured by RayBiotech
Sourced in United States
The Rat ELISA kit is a laboratory equipment designed for the quantitative detection and measurement of target analytes in rat biological samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to provide accurate and reproducible results.
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Lab products found in correlation
7 protocols using rat elisa kit
1
Striatal Biomarker Assessment in Rats
2
Cytokine Measurement Using ELISA
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Serum IL-10 and serum IFN-γ were measured following their manufacturer's instructions using specific rat Elisa kits from RayBiotech.com .
3
Rat Cytokine ELISA Assay
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Enzyme-linked immunosorbent assays (ELISA) were conducted using specific rat IL-13 and IL-4 ELISA kits and manufacturer's directions (RayBiotech, Rat ELISA Kits, USA).
4
Serum and Plasma Biomarker Analysis
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Blood was collected from the caudal vena cava and divided into two tubes for serum and plasma. Serum aspartate aminotransferase (AST) and alanine transaminase (ALT) were measured using commercial kits (Asan Pharmaceutical, Seoul, Korea). The levels of plasma triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) were analyzed by using an autoanalyzer (Hitachi, Tokyo, Japan). The angiotensin-converting enzyme (ACE) level (Cusabio Corporation, Wuhan, China) and angiotensin II level in serum (Raybiotech Inc., Norcross, GA, USA) were measured using the rat ELISA kit.
5
Quantitative Cytokine ELISA in Rat Serum
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Quantitative enzyme-linked immune-sorbent assay (ELISA) of tumor necrosis factor-alpha (TNF-a), interleukins-2, 6 and, 8 were carried out in the serum on a 96-wells microplate using the rat ELISA kit according to the manufacturer's instructions (RayBiotech, USA).
6
Quantification of ROS and IL-6 Levels
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ROS content was determined using the ROS kit from AMSBIO Co., Milton, UK, according to [50 (link)]. The IL-6 levels in the tissue were estimated quantitatively using a RAT ELISA kit from Ray Biotech, Inc. (Norcross, GA, USA).
7
Cytokine Analysis in Rat Model
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Proinflammatory cytokine analysis of TNF‐α and IL‐6 was performed using Rat‐ELISA Kit RayBio®, USA, and protocols werre followed as per the user manual provided.
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