Spherisorb analytical column

Manufactured by Waters Corporation

The Spherisorb analytical column is a high-performance liquid chromatography (HPLC) column manufactured by Waters Corporation. It is designed for analytical separations and provides reliable and consistent performance in a variety of HPLC applications.

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Lab products found in correlation

Ultimate 3000, by Thermo Fisher Scientific (1 mentions) Acquity uplc beh c18 column, by Waters Corporation (1 mentions) Orbitrap elite, by Waters Corporation (1 mentions) Heparinases 1 2 3, by New England Biolabs (1 mentions)

Close spelling variants of this product

Spherisorb column (10 citations) Spherisorb 5 μm ODS2 4.6 × 150 mm analytical column (3 citations) Spherisorb 5 µm ODS2 4.6 × 50 mm analytical column (2 citations) Spherisorb 5 μm ODS2 4.6 × 250 mm analytical column (2 citations)

2 protocols using spherisorb analytical column

Syndecan Heparan Sulfate Disaccharide Analysis

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Syndecan EDs were digested with 1 µL Heparinases I–II–III (New England Biolabs) to break down any potential Heparin/HS into disaccharides. The enzymatic products were then separated with SAX-HPLC (4.6 × 250 mm Waters Spherisorb analytical column with 5 μm particle at 1.0 ml/min flow rate) coupled to fluorescence detection via postcolumn derivatization. The separations of sample disaccharides were compared to the separation of standard disaccharides (Dextra Laboratories) for identification and quantification. LC–MS analysis was performed on a Dionex Ultimate 3000 LC system interface with Thermo Scientific Orbitrap Elite; Separation was carried out on a 2.1 × 150 mm Waters Acquity UPLC BEH C18 column with 1.7 µm particle size at 30 °C. The analytes were monitored by Orbitrap Elite with spray voltage of 3.6 kv and capillary temperature of 275 °C. Mobile phase (A): [20 mM tributylamine acetate in water, pH 4.1], mobile phase (B): [20 mM tributylamine acetate in 80% acetonitrile].

Corti F., Wang Y., Rhodes J.M., Atri D., Archer-Hartmann S., Zhang J., Zhuang Z.W., Chen D., Wang T., Wang Z., Azadi P, & Simons M. (2019). N-terminal syndecan-2 domain selectively enhances 6-O heparan sulfate chains sulfation and promotes VEGFA165-dependent neovascularization. Nature Communications, 10, 1562.

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Disaccharide Composition Analysis of Heparin

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Strong anion exchange high performance liquid chromatography (SAX-HPLC) was performed at the University of Georgia Complex Carbohydrate Research Center (CCRC) to determine the disaccharide composition of Hep and Hep^−N. Hep and Hep^−N were dissolved at 12.5 mg/mL in a heparinase mixture of 0.5 U/mL heparinases I, II, and III for 24 hours at 37°C. The reaction was then quenched by heating the mixture for 2 mins at 100°C.
SAX-HPLC was carried out on an Agilent system using a Waters Spherisorb analytical column (4.6×250 mm; 5 μm particle size) at 25°C. Analytes were detected by their UV absorbance at 232 nm using a buffer system consisting of 2.5 mM sodium phosphate (Na₃PO₄) and pH 3.5, which was gradually transitioned from 0 to 1.2 M NaCl. The flow rate was 1.0 mL/min and detection was performed by post-column derivatization and fluorescence detection. Commercial standard disaccharides (Dextra Laboratories) were used for identification of each disaccharide based on elution time and calibration.

Tellier L.E., Krieger J.R., Brimeyer A.L., Coogan A.C., Falis A.A., Rinker T.E., Schudel A., Thomas S.N., Jarrett C.D., Willett N.J., Botchwey E.A, & Temenoff J.S. (2018). Localized SDF-1α Delivery Increases Pro-Healing Bone Marrow-Derived Cells in the Supraspinatus Muscle Following Severe Rotator Cuff Injury. Regenerative engineering and translational medicine, 4(2), 92-103.

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