Pyromark rt kit

Manufactured by Qiagen

Sourced in United States

The PyroMark RT kit is a laboratory equipment product designed for real-time PCR analysis. It provides the necessary reagents and tools to perform quantitative gene expression analysis.

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Lab products found in correlation

Ez rna methylation kit, by Zymo Research (2 mentions) Pyromark q24 system, by Qiagen (1 mentions)

Spelling variants of this product

PyroMark kit (18 citations) Pyromark OneStep RT-PCR kit (2 citations)

2 protocols using pyromark rt kit

RNA Bisulfite Conversion and Pyrosequencing

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One μg of RNA was bisulfite converted with the EZ RNA Methylation Kit (Zymo Research Irvine, US; R5001), and reverse transcribed with the QIAGEN PyroMark RT kit 978801, using an optimized pair of one biotinylated and unmodified primer. Ten microliters of cDNA were enriched with Streptavidin-coupled sepharose beads, and pyro-sequencing was performed on a PyroMark Q24 system (QIAGEN; 9001514) according to manufacturer’s instructions.

Rosenberger F.A., Tang J.X., Sergeant K., Moedas M.F., Zierz C.M., Moore D., Smith C., Lewis D., Guha N., Hopton S., Falkous G., Lam A., Pyle A., Poulton J., Gorman G.S., Taylor R.W., Freyer C, & Wredenberg A. (2022). Pathogenic SLC25A26 variants impair SAH transport activity causing mitochondrial disease. Human Molecular Genetics, 31(12), 2049-2062.

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RNA Bisulfite Conversion and Pyrosequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols

One microgram of RNA per sample was bisulfite converted with the EZ RNA Methylation Kit (Zymo Research) and transcribed into cDNA and PCR amplified with a PyroMark RT kit (QIAGEN) using an optimized pair of one biotinylated and one unmodified primer. cDNA amplicons were enriched with streptavidin-coupled sepharose beads before following the manufacturer’s recommendations for pyrosequencing.

Schober F.A., Moore D., Atanassov I., Moedas M.F., Clemente P., Végvári Á., Fissi N.E., Filograna R., Bucher A.L., Hinze Y., The M., Hedman E., Chernogubova E., Begzati A., Wibom R., Jain M., Nilsson R., Käll L., Wedell A., Freyer C, & Wredenberg A. (2021). The one-carbon pool controls mitochondrial energy metabolism via complex I and iron-sulfur clusters. Science Advances, 7(8), eabf0717.

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