Mouse ifn alpha all subtype elisa kit

Manufactured by PBL Assay Science

The Mouse IFN Alpha All Subtype ELISA Kit is a laboratory assay used for the quantitative measurement of interferon alpha (IFN-α) levels in mouse biological samples. The kit employs the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify the presence of this cytokine.

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Lab products found in correlation

2 3 cgamp elisa kit, by Cayman Chemical (1 mentions) Recombinant mouse ifn β, by PBL Assay Science (1 mentions) Control rabbit igg, by Santa Cruz Biotechnology (1 mentions) Verikine hs mouse ifn beta elisa kit, by PBL Assay Science (1 mentions) Mojosort mouse cd45 nanobeads, by BioLegend (1 mentions) M csf, by Thermo Fisher Scientific (1 mentions) Murine tnf α, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Mouse IFN Alpha All Subtypes High Sensitivity ELISA kit (2 citations)

2 protocols using mouse ifn alpha all subtype elisa kit

Quantification of IFNα and cGAMP

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Cells were cultured as described in section of Cell Preparation. After collecting the samples, centrifugation was performed. The supernatant was used as the sample for IFNα and the cells were used as the sample for cGAMP. The concentration of IFNα or cGAMP was assessed by Mouse IFN Alpha All Subtype ELISA Kit (PBL Assay Science) or by 2′3′ – cGAMP ELISA kit (Cayman) as according to the manufacture’s protocol.

Kanno T., Nakajima T., Yokoyama S., Asou H.K., Sasamoto S., Kamii Y., Hayashizaki K., Ouchi Y., Onodera T., Takahashi Y., Ikeda K., Hasegawa Y., Kinjo Y., Ohara O., Nakayama T, & Endo Y. (2021). SCD2-mediated monounsaturated fatty acid metabolism regulates cGAS-STING-dependent type I IFN responses in CD4+ T cells. Communications Biology, 4, 820.

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Murine Cytokine and IFN-beta Assays

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Murine TNF-α and M-CSF were purchased from Peprotech. Recombinant mouse IFN-β was from PBL Assay Science. The control IgG (rabbit) was obtained from Santa Cruz Biotechnology, and IFN-β neutralizing antibody (rabbit polyclonal antibody against mouse interferon-beta) was from PBL Assay Science. Mouse IFN Alpha All Subtype ELISA Kit and VeriKine-HS Mouse IFN Beta ELISA Kit were purchased from PBL Assay Science. MojoSort Mouse CD45 Nanobeads were obtained from Biolegend.

Deng Z., Ng C., Inoue K., Chen Z., Xia Y., Hu X., Greenblatt M., Pernis A, & Zhao B. (2020). Def6 regulates endogenous type-I interferon responses in osteoblasts and suppresses osteogenesis. eLife, 9, e59659.

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