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Phospho erk clone d13.14.4e

Manufactured by Cell Signaling Technology
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Phospho-ERK (clone D13.14.4E) is a rabbit monoclonal antibody that recognizes the phosphorylated forms of Extracellular Signal-Regulated Kinase (ERK1/2). ERK1/2 are serine/threonine protein kinases that play a key role in the Ras-Raf-MEK-ERK signal transduction pathway, which is involved in the regulation of cell growth, proliferation, and differentiation.

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Lab products found in correlation

Alexa fluor 488, by Cell Signaling Technology (1 mentions) Phospho akt clone d9e, by Cell Signaling Technology (1 mentions) Phospho met clone d26, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Phospho-ERK (527 citations) Clone D13.14.4E (4 citations) ERK/phospho-ERK (3 citations)

2 protocols using phospho erk clone d13.14.4e

1

Phospho-Protein Induction Quantification

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Phospho-protein induction in permeabilized cells was determined using Alexa Fluor 647-, phycoerythrin-, Alexa Fluor 488- or biotin-labelled antibodies to phospho-ERK (clone D13.14.4E, diluted 1:1,000), phospho-p38 (clone 3D7, diluted 1:1,000), phospho-JNK (clone G9, diluted 1:1,000) or phospho-IκBα (clone 14D4, diluted 1:1,000; all Cell Signaling) by flow cytometry.
Lahiri A., Hedl M., Yan J, & Abraham C. (2017). Human LACC1 increases innate receptor-induced responses and a LACC1 disease-risk variant modulates these outcomes. Nature Communications, 8, 15614.
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2

Western Blot Analysis of Signaling Proteins

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Western blotting was performed as previously described (25 (link)). Primary antibodies for phospho-ERK (clone D13.14.4E; catalog #4370S), total ERK (clone 137F5; catalog #4695S), phospho-AKT (clone D9E; catalog #4060S), total AKT (clone 40D4; catalog #2920S), phospho-Met (clone D26; catalog #3077S), and total Met (clone D1C2; catalog #8198S) from Cell Signaling Technology.
Phadke M.S., Chen Z., Li J., Mohamed E., Davies M.A., Smalley I., Duckett D.R., Palve V., Czerniecki B.J., Forsyth P.A., Noyes D., Adeegbe D.O., Eroglu Z., Nguyen K.T., Tsai K.Y., Rix U., Burd C.E., Chen Y.A., Rodriguez P.C, & Smalley K.S. (2021). Targeted therapy given after anti-PD-1 leads to prolonged responses in mouse melanoma models through sustained antitumor immunity. Cancer immunology research, 9(5), 554-567.
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