The largest database of trusted experimental protocols

Celltiter 96tm aqueous one solution cell proliferation assay

Manufactured by Thermo Fisher Scientific
Sourced in United States

The CellTiter 96TM AQueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation or cytotoxicity assays. It utilizes a tetrazolium compound that is bioreduced by cells into a colored formazan product, which can be quantified by absorbance measurements.

Automatically generated - may contain errors

2 protocols using celltiter 96tm aqueous one solution cell proliferation assay

1

Synthesis and Characterization of Boronic Acid-Dextran Conjugate

Check if the same lab product or an alternative is used in the 5 most similar protocols
4-(Hydroxymethyl) phenylboronic acid pinacol ester (PBAP), dextran with molecular weight (MW) of 5000 g/mol, and ferrous perchlorate were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd (Shanghai, China). 4-Dimethylaminopyridine (DMAP) and NaClO were purchased from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). N,N’-Carbonyldiimidazole (CDI) was purchased from J&K Chemical Co., Ltd. (Shanghai, China). RAP was purchased from Dalian Meilun Biotechnology Co., Ltd (Dalian, China). Deuterated dimethyl sulfoxide (DMSO-d6) and deuterated methanol (methanol-d4) were purchased from Sun Chemical Technology Co., Ltd (Shanghai, China). Hydrogen peroxide (H2O2) was purchased from ChengDu Chron Chemicals Co., Ltd (Chengdu, China). DAPI and cell total protein extraction kits were purchased from Beyotime Institute of Biotechnology Co., Ltd (Jiangsu, China). 1,19-Dioctadecyl-3,3,39,39-tetramethylindodicarbocyanine perchlorate (DiD) was purchased from Biotium Inc. (Fremont, USA). The CellTiter 96TM AQueous One Solution Cell Proliferation Assay (MTS) was purchased from Thermo Fisher Scientific (SanJose, CA, USA).
+ Open protocol
+ Expand
2

Nanoparticle Fabrication and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
RAP and PLGA (MW 90000, 50:50) were purchased from Dalian Meilun Biotechnology Co., Ltd. (Dalian, Chia). 1,19-Dioctadecyl-3,3,39,39-tetramethylindodicarbocyanine perchlorate (DiD) was purchased from Biotium Inc. (Fremont, US). DiO, DAPI, Cell Total Protein Extraction kits and Membrane Protein Extraction kits were supplied by Beyotime Institute of Biotechnology (Jiangsu, China). The CellTiter 96TM AQueous One Solution Cell Proliferation Assay (MTS) was purchased from Thermo Fisher Scientific (SanJose, CA, USA). Lipopolysaccharide (LPS) was purchased from Solarbio (Beijing, China). The mouse glycoprotein ELISA kit, mouse TNF-α ELISA kit and mouse IL-6 ELISA kit were purchased from Wuhan Colorful Gene Biological Technology Co., Ltd. (Wuhan, China). LysoTracker Green was purchased from Yeasen Biotech Co. Ltd. (Shanghai China). Ultrapure water with a resistivity of 18.2 MΩ·cm was used throughout the experiments.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!