Af3117

The following antibodies were used in immunostaining experiments. Primary antibodies: for EWI-2 a goat polyclonal-antibody (AF3117; R&D Systems, Minneapolis, USA), for CD81 a rabbit polyclonal antibody (sc-9158 H-121, Santa Cruz, Texas, USA) and a mouse monoclonal antibody (sc-7636 1.3.3.22, Santa Cruz), for CD9 a mouse monoclonal antibody (MM2-57 CBL162, Merck, Darmstadt, Germany), for pERM a rabbit monoclonal antibody (mAB 3726T, Cell Signaling Technology, Massachusetts, USA), for CD151 a mouse monoclonal antibody (Biorad MCA1856GA) and for CD44 a rabbit polyclonal antibody (15675-1-AP, ProteinTech, Rosemont, USA).
As secondary antibodies we used: STAR RED coupled to donkey-anti-goat (STRED-1055-500UG, Abberior, Göttingen, Germany), goat-anti-rabbit (STRED-1002-500UG, Abberior), and goat-anti-mouse (STRED-1001-500UG, Abberior). AlexaFluor™ 594 coupled to donkey-anti-mouse (A21203, Invitrogen™, Carlsbad, USA), donkey-anti-goat (A11058, Invitrogen™) and donkey-anti-rabbit (A21207, Invitrogen™). Moreover, we used nanobodies GFP booster Atto488 (Alpaka nanobody Gba488-100, ChromoTek, Rosemont, USA) and GFP booster Atto594 (Alpaka nanobody Gba594-100, ChromoTek).

The following antibodies were used for immunofluorescence: guinea pig anti-insulin (A056401, Dako, 1:1000), rabbit anti-phospho-CREB (Ser133) (9198, Cell Signaling, 1:250), rabbit anti-Oxytocin (T-4084, Peninsula Laboratories, 1:1000), mouse anti-V5 tag (R960-25, Invitrogen, 1:250), goat anti-guinea pig conjugated to Alexa Fluor 488 (A11073, Invitrogen, 1:500), goat anti-rabbit conjugated to Cy5 (A10523, Invitrogen, 1:500), and goat anti-mouse conjugated to Alexa647 (115-605-003, Jackson ImmunoResearch, 1:500). The following antibodies were used for Western blot: mouse anti-V5 tag (R960-25, Invitrogen, 1:5000), goat anti-IgSF8 (AF3117, R&D Systems, 1:500), mouse anti-Alpha-synuclein (610786, BD Biosciences, 1:500), rabbit anti-Beta-arrestin (ab32099, Abcam, 1:1000), rabbit anti-c-Raf (53745, Cell Signalling, 1:500), rabbit anti-GAPDH (2118, Cell Signalling, 1:1000), goat anti-rabbit conjugated to HRP (401393, Sigma, 1:10,000), rabbit anti-goat conjugated to HRP (401515, Sigma, 1:10,000), and goat anti-mouse conjugated to HRP (401253, Sigma, 1:10,000).