Lc 10advp solvent delivery system

Dried ground root bark of M. alba (10 g) was soaked exhaustively with cold water (0.5 L) for 1 h. Subsequently the material was decocted for 20 min in a pot with a lid. After filtration through cotton wool, the decoction procedure was repeated with the remaining plant material. The filtrated decoctions were combined (MAD) and an aliquot was used for HPLC analyses. HPLC of MAE, MAF, and MAD (Supplementary Fig. 1) was performed on a Shimadzu device consisting of an LC-10ADVP solvent delivery system, a FCV-10ALVP low-pressure gradient flow control valve, an SCL-10AVP system controller, a DGU-14A degasser, and a SPD-M20A photodiode array (PDA) detector. LC-parameters: stationary phase: Agilent Zorbax SB-C18 3.5 μm (150 × 4.6 mm); temperature: 25 °C; mobile phase: A = water; B = acetonitrile; flow rate 1.0 mL/min; PDA detection wavelength: 205 nm; injection volume: 10 μL; Separations were performed by gradient elution (70/30 A/B in 5 min to 55/45 A/B, then within 15 min to 45/55 A/B, and within 2 min to 2/98 A/B), followed by a 10 min column wash (2A/98B) and a re-equilibration period of 10 min. All chemicals and solvents used were analytical grade.