Quickvue dipstick strep a

Manufactured by Quidel

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The QuickVue Dipstick Strep A is a rapid diagnostic test used to detect the presence of Streptococcus pyogenes (Group A Streptococcus) in throat swab specimens. The test provides qualitative results, indicating the presence or absence of the target antigen.

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QuickVue (23 citations)

4 protocols using quickvue dipstick strep a

Evaluation of Rapid Antigen Detection Tests for GAS

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The RADT kit for GAS used in Kronoberg County during the study period was QuickVue Dipstick Strep A (Quidel Corporation, San Diego, CA, USA), a lateral-flow immunoassay using antibody-labelled particles [21 (link)]. The test detects viable and nonviable organisms directly from throat swabs.
Routine throat cultures for the recovery of large colony β-haemolytic streptococci used standard procedures, as previously described [9 (link)]. Starting in 2013, the laboratory also offered an extended throat culture that added an anaerobic plate for the recovery of F. necrophorum [9 (link)]. In late 2013, with the introduction of matrix-assisted laser desorption/ionization with time-of-flight mass spectrometer (MALDI-TOF), the reporting of streptococci transitioned from Lancefield classification to species identification. As a result, GAS was reported as S. pyogenes and most group C and G streptococci were reported as SDSE. In this study, group C or G streptococci were reported as SDSE. During the study period, before the transition, group C and G streptococci constituted 40% of all β-haemolytic streptococci in throat cultures; after the transition, the corresponding proportion for SDSE was 42% (data not shown).

Pallon J., Sundqvist M., Rööst M, & Hedin K. (2021). Association between bacterial finding, antibiotic treatment and clinical course in patients with pharyngotonsillitis: a registry-based study in primary healthcare in Sweden. BMC Infectious Diseases, 21, 779.

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Comprehensive Respiratory Pathogen Screening

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As previously described [14 (link)], all patients and controls were sampled from the nasopharynx, throat and blood and screened with routine culture for β-haemolytic streptococci (Lancefield group A, C, and G); with anaerobic culture for Fusobacterium necrophorum; with serology for Epstein–Barr virus; with single PCR for Influenza A and B viruses and Mycoplasma pneumoniae; and with multiplex real-time PCR for two intracellular bacteria and 13 viruses: M.pneumoniae, Chlamydophila pneumoniae, Adenovirus, Bocavirus, Coronavirus NL63, Coronavirus OC43, Coronavirus HKU1, Coronavirus 229E, Enterovirus, Influenza A virus, Influenza B virus, Metapneumovirus, Parainfluenzavirus, Rhinovirus and Respiratory syncytial virus. The primers and probes used in the multiplex PCR have been described elsewhere [17 (link)].
RADTs for GAS are routinely used at most Swedish primary health care centres. The only RADT kit available in Region Kronoberg during the study period was QuickVue Dipstick Strep A (Quidel Corporation, San Diego, CA, USA), a lateral-flow immunoassay using antibody-labelled particles. The test detects either viable or nonviable organisms directly from throat swabs.

Pallon J., Rööst M., Sundqvist M, & Hedin K. (2021). The aetiology of pharyngotonsillitis in primary health care: a prospective observational study. BMC Infectious Diseases, 21, 971.

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Rapid Antigen Detection Test for Group A Streptococcus

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At inclusion all patients were clinically examined by a physician. A case report form was completed covering the Centor criteria, clinical signs, age, gender and the allocated treatment regimen (5 or 10 days). Throat swabs for RADT and culture were performed at the inclusion visit. To reduce discomfort for children a double swab method was used, rotating the two swabs towards the tonsils and pharynx at one time. All patients were offered a single follow-up visit with a physician within 5–7 days after completion of the antibiotic treatment. Due to preferences from participants some follow-up visits took place earlier or later on than 5–7 days after completed antibiotic treatment. The follow-up visit included an examination by a physician evaluating clinical cure, and throat swabs were obtained for RADT and culture for GAS.
The RADT was performed according to instructions from the manufacturer with the locally available equipment. The tests used were either a OSOM Strep A test manufactured by Sekisui Diagnostics, or a QuickVue DipStick Strep A test manufactured by Quidel. Both are RADT immunoassay tests for GAS. Swabs for throat culture were sent to regional microbiological laboratories for culture on blood agar plates incubated overnight at 35–37 °C.

Rystedt K., Hedin K., Tyrstrup M., Skoog-Ståhlgren G., Edlund C., Giske C.G., Gunnarsson R, & Sundvall P.D. (2023). Agreement between rapid antigen detection test and culture for group A streptococcus in patients recently treated for pharyngotonsillitis - a prospective observational study in primary care. Scandinavian Journal of Primary Health Care, 41(1), 91-97.

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Rapid Diagnostic Tests in Swedish PHC

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RADTs and CRP tests are widely used in Swedish PHC and are readily available at most PHC centres. In Region Kronoberg, the RADT kit used during the study period was Quick-Vue Dipstick Strep A (Quidel Corporation, San Diego, CA, USA), a lateral-flow immunoassay using antibody labelled particles. The CRP test used during the study period was Afinion™ CRP assay (Abbott Laboratories, Abbott Park, Illinois, USA), a rapid in vitro diagnostic test for quantitative determination of CRP with a measuring range of 5–200 mg/l and an assay time of 3–4 min.

Pallon J., Sundqvist M, & Hedin K. (2024). The use and usefulness of point-of-care tests in patients with pharyngotonsillitis – an observational study in primary health care. BMC Primary Care, 25, 15.

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