BCL11B/CTIP2 expression in the aorta was probed using five antibodies that recognize different epitopes on CTIP2: anti-CTIP2 rat monoclonal (Abcam, ab18465), rabbit polyclonal (Abcam, ab70452), rabbit polyclonal (Abcam, ab28448), rabbit monoclonal (Abcam, ab187668), and rabbit monoclonal (CST signaling 12120). Western blot and IHC staining antibody dilutions were used as recommended by the manufacturer. CD45 expression in the aorta was probed with anti-CD45 rabbit polyclonal (Abcam, ab10558) at 1:2000 dilution. Beta-actin rabbit polyclonal immunoglobulin G (ThermoFisher Scientific) was used for the western blot loading control at 1:1000 dilution. Donkey anti-rabbit (LI-COR) IRDye® 800CW and goat anti-rat (ThermoFisher Scientific) Alexa Fluor® 680-conjugated secondary antibodies were used at 1:5000 dilution for western blot.
Ab187668
Manufactured by Abcam
Ab187668 is a highly specific monoclonal antibody designed to detect and bind to a target protein. The antibody is suitable for use in various immunoassays and research applications, but a detailed description of its intended use or function is not available.
Automatically generated - may contain errors
2 protocols using ab187668
1
Probing CTIP2 and CD45 in Aorta
2
Aortic BCL11B/CTIP2 and CD45 Expression
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