Oasis mcx spe cartridge

Manufactured by Waters Corporation

Sourced in United States

Oasis MCX SPE cartridges are solid-phase extraction (SPE) products designed for sample preparation. They consist of a mixed-mode sorbent that can retain both acidic and basic analytes. The cartridges are intended for use in analytical sample preparation workflows.

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Lab products found in correlation

Turbovap lv, by Biotage (1 mentions) Lc ms grade formic acid, by Merck Group (1 mentions) Mdma d5, by Cerilliant (1 mentions) Milli q system, by Merck Group (1 mentions) Ammonium hydroxide, by Merck Group (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Lc ms grade methanol, by Merck Group (1 mentions) Acquity uplc h class system, by Waters Corporation (1 mentions) Targetlynx v4, by Waters Corporation (1 mentions) Acquity uplc beh c18 column, by Waters Corporation (1 mentions)

Close spelling variants of this product

MCX Oasis cartridges Oasis MCX cartridges MCX SPE cartridge Oasis MCX MCX cartridges Oasis cartridges SPE cartridges

6 protocols using oasis mcx spe cartridge

Mouse Plasma Protein Extraction Protocol

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The mouse plasma samples were subjected to protein precipitation and solid phase extraction (SPE). To remove plasma proteins,

10 μ L

of the IS working solution (

500 ng / mL

) was added to

100 μ L

of plasma,

800 μ L

of water, and

100 μ L

of 25% (wt/vol) trichloroacetic acid (TCA), and the mixture vortexed for 10 min. For SPE, the supernatant from protein precipitation was loaded onto an OASIS MCX SPE cartridge (Waters Corporation), which was preconditioned with

1 mL

of methanol and equilibrated with

1 mL

of water and allowed to flow by gravity. The cartridge was washed twice with

1 mL

of 5% methanol, 5% formic acid in water (v/v), followed by vacuum drying for 5 min. Analytes were eluted with

1 mL

of fresh 20% methanol, 5% ammonia in water (v/v). The eluent was evaporated to complete dryness in a centrifuge evaporator at 50°C. The lyophilized sample was reconstituted with

100 μ L

of 95% ACN/water (v/v, 9/1) with 2% formic acid prior to injection.

Raber J., Perez R., Torres E.R., Krenik D., Boutros S., Patel E., Chlebowski A.C., Torres E.R., Perveen Z., Penn A., Paulsen D.B., Bartlett M.G., Jia E., Holden S., Hall R., Morré J., Wong C., Ho E., Choi J., Stevens J.F., Noël A., Bobe G, & Kisby G. (2021). Effects of Chronic Secondhand Smoke (SHS) Exposure on Cognitive Performance and Metabolic Pathways in the Hippocampus of Wild-Type and Human Tau Mice. Environmental Health Perspectives, 129(5), 057009.

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Clonidine Oral Liquid Analysis by HPLC

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Phosphoric acid 0.1% in water (1.0 mL) was added to the 5 mL-centrifuge tubes containing the clonidine oral liquid samples (1.0 mL). Tubes were vigorously vortexed (20 s) and then centrifuged (2135 g, 15 min). This resulted in a phase separation: part of the vehicle and tablet excipients precipitated. An Oasis MCX SPE cartridge (1 mL/30 mg, Waters, MA, USA) attached to a Cerex 48 positive pressure SPE apparatus (Cerex, GA, USA) was loaded with 800 μL of supernatant. The cartridge was first washed with ammonium formate 100 mM with 2% formic acid in water (1.0 mL) and then with methanol (1.0 mL). Ammonium hydroxide 6% in methanol (1.0 mL) was used for the elution. Eluates were collected in glass tubes and evaporated to dryness in a water bath set at 40°C under a stream of nitrogen gas (TurboVap LV, Biotage, Uppsala, SE). Residues were reconstituted in mobile phase A (200 μL) and injected in duplicate into the HPLC system (Fig 1).

Coache D., Friciu M., Roullin V.G., Boulé M., Forest J.M, & Leclair G. (2021). Stability evaluation of compounded clonidine hydrochloride oral liquids based on a solid-phase extraction HPLC-UV method. PLoS ONE, 16(11), e0260279.

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Quantitative Analysis of Illicit Drugs

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Benzoylecgonine, amphetamine, methamphetamine and MDMA, as well as the deuterated compounds that were used as internal standards, MDMA-d5 (0.1 mg/mL), methamphetamine-d5, amphetamine-d5 and benzoylecgonine-d3, were supplied by Cerilliant (Round Rock, TX, USA) in the form of 1 mg/mL solution in methanol or acetonitrile. Working solutions were prepared in methanol. LC-MS grade methanol, LC-MS grade formic acid (98 %) and hydrochloric acid (35 %) were purchased from Merck (Darmstadt, Germany). Ammonium hydroxide (25 %) was acquired from Sigma-Aldrich (Steinhem, Germany), while the ultrapure water used was from a Milli-Q system (Millipore). Nitrogen (99.9995 % purity) for sample drying was supplied by Linde Ltd. (Cyprus). Oasis MCX SPE cartridges (60 mg, 3 cc) were purchased from Waters Corporation (Milford, MA, USA).

Psichoudaki M., Mina T., Savvidou M., Mina C., Michael C, & Fatta-Kassinos D. (2022). Wastewater-based monitoring of illicit drugs in Cyprus by UPLC-MS/MS: The impact of the COVID-19 pandemic. The Science of the Total Environment, 854, 158747.

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Quantifying Adduct Uptake in HUVECs

Cited 2 times

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HUVECs were plated on 6-well plates at a density of 30,000 cells/cm² and treated the next day with 200 ng/mL of isotopically labeled MG adducts (¹⁵N₅-R,S-CEdG or ¹⁵N₅-R,S-CEG). At each indicated timepoint, conditioned media was collected, centrifuged to remove cellular debris, and processed via cation exchange solid-phase extraction using Oasis MCX SPE cartridges (Waters, Milford, MA, USA) [9 (link),23 (link),24 (link)]. Additionally, cell pellets were collected and lysed using 10% w/v trichloroacetic acid, and cellular debris was separated via centrifugation for 15 min at 4 °C at 12,000× g. Both the cell pellets and conditioned media were spiked with isotopically labeled internal standards prior to their quantification via LC-MS as previously described [23 (link),24 (link)]. To quantify adduct uptake, cells treated with ¹⁵N_5-R,S-CEdG used ¹⁵N₅-S-CEG at a final concentration of 5 ng/mL as an internal standard. Conversely, cells treated with ¹⁵N_5-R,S-CEG used ¹⁵N₅-S-CEdG at a final concentration of 5 ng/mL as an internal standard.

Lai S.W., Bhattacharya S., Lopez Gonzalez E.D, & Shuck S.C. (2024). Methylglyoxal-Derived Nucleoside Adducts Drive Vascular Dysfunction in a RAGE-Dependent Manner. Antioxidants, 13(1), 85.

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Plasma Sample Preparation by PPT and SPE

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Sample preparation was carried out by a combined method of protein precipitation (PPT) and solid-phase extraction (SPE). This method was based on a method published by Li et al, while steps with different conditions or parameters were specially noted here.[30 ]
To each 50 μL of plasma, 10 μL of IS working solution (500.0 ng/mL), 90 μL of 25% (w/v) TCA and 850 μL of water were sequentially added. The mixture was vortexed for 10 min and then centrifuged at 4500 × g for 10 min for PPT. SPE based on mixed-mode cation exchange mechanism was used as the second step of sample preparation, in which Oasis MCX SPE cartridges from Waters (Milford, MA) were used. Processes including conditioning, sample loading, washing and eluting were identical with the published method.[30 ]

Li P., Beck W.D., Callahan P.M., Terry AV J.r, & Bartlett M.G. (2014). Pharmacokinetics of cotinine in rats: a potential therapeutic agent for disorders of cognitive function. Pharmacological reports : PR, 67(3), 494-500.

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Quantification of Plant Hormones

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Plant samples frozen in liquid nitrogen were grounded using motor and pestle. Around 200 mg of samples (shoot or root) was measured from ground powder and extracted with ice cold methanol/water/formic acid (15/4/1 v/v/v) added with isotope labelled internal standards (IS).
Similar concentrations of internal standards (IS) of ABAs, auxins and gibberellins were added into samples and calibration standards. The samples were purified using Oasis MCX SPE cartridges (Waters) according to manufacturer's protocol. The samples were injected on Acquity UPLC BEH C18 column (1.7 µm, 2.1x100 mm, Waters; with gradients of 0.1% acetic acid in water or acetonitrile), connected to Acquity UPLC H class system (Waters) coupled with UPLC-ESI-MS/MS triple quadrupole mass spectrometer for identification and quantification of hormones.
The hormones were measured using MS detector, both in positive and negative mode, with two MRM transitions for each compound. External calibration curves were used for quantification, and calculated through Target Lynx (v4.1; Waters) software by comparing the ratios of MRM peak areas of analyte to that of internal standard. Heat map was generated using the BAR HeatMapper Plus tool (http://bar.utoronto.ca/ntools/cgi-bin/ntools_heatmapper_plus.cgi).

Zhang Y., Kilambi H.V., Liu J., Bar H., Lazary S., Egbaria A., Ripper D., Charrier L., Aharoni A., Ragni L., Strader L.C., Sade N., Weinstain R., Geisler M., & Shani E. (2021). ABA homeostasis and long-distance translocation is redundantly regulated by ABCG ABA importers.

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