Pelp1 antibody

TNBC model cells (MDA-MB-231 and MDA-MB-468) were purchased from the American-Type Culture Collection (ATCC) and maintained in RPMI 1640 and DMEM medium supplemented with 10% fetal bovine serum, respectively (Sigma-Aldrich, St. Louis, MO). HEK-293T cells were obtained from ATCC and maintained in DMEM supplemented with 10% fetal bovine serum. MDA-MB-231 cells expressing control- or PELP1-shRNA were generated as described earlier (22 (link)). Non-targeted and SMARTpool PELP1siRNA were obtained from Dharmacon Inc. (Lafayette, CO). The p53 (mouse monoclonal) and E2F1 mouse monoclonal antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). The PELP1 antibody was purchased from Bethyl laboratories (Montgomery, TX). Cleaved Caspase-3, cleaved PARP-1, and p53 antibody was purchased from Cell Signaling Technologies (Beverly, MA). The phospho-PELP1 antibody was generated as described previously (24 (link)). GFP-TRAP beads were obtained from ChromoTek (Planegg-Martinsried, Germany). Cisplatin (cis-Diamineplatinum (II) dichloride), carboplatin, and camptothecin were purchased from Sigma-Aldrich (St. Louis, MO, USA). For the radiation treatment, the exponentially growing cells were exposed to various doses of ¹³⁷Cs -rays at a dose rate of 0.93 Gy/min at room temperature using a Gamma Cell-40 irradiator (Atomic Energy of Canada Ltd, Montreal, Canada).