96 maxisorp elisa plate

GMC101 and N2 worms were incubated on FUDR plates until days 0, 1, 3, 5, 7, and 10 of adulthood, according to what is reported in the text for each experiment. At a specific time the worms were washed out of the plates and snap-frozen using liquid nitrogen. For sample preparation, around 1,000 animals were thawed and resuspended in 500 μL PBS supplemented with EDTA-free protease inhibitor tablets 1× (Roche), after which they were sonicated (5 × 45 s, 50% cycles, 50% maximum power on ice) using a Bandelin Sonopuls HD 2070. After sonication, the lysates were centrifuged at maximal speed using a bench centrifuge and 20 μL of supernatant (5 μg) were loaded on a 96 Maxisorp ELISA plate (Nunc) with no shaking overnight at 4 °C. ELISAs were conducted as described above (ELISA-Based Screening of the Antibodies).

Thirty-microliter aliquots (5 μg) were immobilized on a 96 Maxisorp ELISA plate (Nunc) with no shaking overnight at 4 °C. Experiments were then performed as described above (ELISA-Based Screening of the Antibodies).