Mouse anti claudin 7

Immunostaining of NHP jejunum sections was performed as described elsewhere [29 (link),52 (link)]. Deparaffinated sections from the jejunum were rehydrated and then incubated with rabbit anti-claudin-2 (1:100), rabbit anti-Occludin (1:500), rabbit anti-β-Catenin (1:2000) (all from cell signaling technology, Danvers, MA, USA), rat anti-Ki-67 (1:200), mouse anti-Zona-1(1:450), mouse anti-claudin-7 (1:800), and mouse anti-E-Cadherin (1:2500) (Invitrogen, Carlsbad, CA, USA) in blocking buffer. The primary antibodies were detected using donkey anti-rabbit/rat secondary IgG-AlexaFluor 594 conjugates/donkey anti-mouse Alexafluor 488 conjugate/goat anti-rabbit Alexafluor 488 conjugate/goat anti-mouse Alexafluor 594 conjugate (1:800, Invitrogen, Carlsbad, CA, USA). The sections were then counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Thermo Fisher, Waltham, MA, USA) to visualize cell nuclei and mounted with Prolong^® Gold Antifade Mountant (Invitrogen, Carlsbad, CA, USA). The images were captured and analyzed as described earlier [29 (link)].