PE-conjugated anti-CD3 (17A2), anti-CD14 (rmC5-3), anti-CD16/32 (2.4G2), anti-B220 (RA3-6B2), peridinin-chlorophyll protein (PerCP)-conjugated anti-CD44 (IM7), and allophycocyanin (APC)-conjugated anti-CD25 (PC61) antibodies were purchased from BD Biosciences (San Jose, CA). A cysteine protease inhibitor (E64) and uricase from Arthrobacter globiformis were purchased from Sigma-Aldrich (St. Louis, MO). Endotoxin-free OVA [<0.5 endotoxin unit (EU)/mg protein] was purified from specific pathogen-free chicken eggs under sterile conditions. Recombinant mouse IL-33 (Ser109-Ile266, <0.01 EU/μg protein) was purchased from R&D Systems (Minneapolis, MN). Monosodium urate (MSU) crystals were purchased from Sigma-Aldrich, suspended in PBS at 20 mg/ml, and sonicated for 20 min in an ultrasonic cleaner (BRANSON 2200, Branson Ultrasonics, Danbury, CT) before use. The endotoxin levels in the MSU crystal suspension were less than 0.005 EU/ml. Bromelain (from pineapple stem) and papain (from Carica papaya) were purchased from Sigma-Aldrich and EMD Millipore (Billerica, MA), respectively. Alternaria alternata culture filtrate extract, Aspergillus fumigatus extract, and HDM extract were obtained from Greer Laboratories (Lenoir, NC); these extracts contained less than 2 EU/mg protein.
Bromelain from pineapple stem
Manufactured by Merck Group
Sourced in United States, Germany
Bromelain is a mixture of enzymes derived from the stem of pineapples (Ananas comosus). It exhibits proteolytic activity, which means it can break down proteins. Bromelain is commonly used as a laboratory reagent for various applications, such as protein digestion and cell culture studies.
Automatically generated - may contain errors
Lab products found in correlation
Papain from carica papaya, by Merck Group (1 mentions)
Monosodium urate msu crystals, by Merck Group (1 mentions)
Branson 2200, by Emerson (1 mentions)
Aspergillus fumigatus extract, by Stallergenes Greer (1 mentions)
Hdm extract, by Stallergenes Greer (1 mentions)
Potassium persulfate, by Merck Group (1 mentions)
Coomassie brilliant blue, by Merck Group (1 mentions)
Cellulase from trichoderma reesei, by Merck Group (1 mentions)
1 2 bis dimethylamino ethane, by Merck Group (1 mentions)
Folin ciocalteu s phenol reagent, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
Sodium carbonate, by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Acrylamide, by Merck Group (1 mentions)
2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid diammonium salt, by Merck Group (1 mentions)
Trichloroacetic acid tca, by Samchun (1 mentions)
Sodium acetate trihydrate, by Merck Group (1 mentions)
Calcium acetate, by Merck Group (1 mentions)
4 protocols using bromelain from pineapple stem
1
Multiparameter Immunophenotyping of Murine Cells
2
Enzymatic Hydrolysis of Chlorella Powder
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C. pyrenoidosa powder (CP) used in the experiment was purchased from Jeongwoodang Co., Ltd. (Seoul, Republic of Korea). Microbial protease from Bacillus spp. (EC 3.4.21.14), papain from Carica papaya fruit (EC 3.4.22.2), bromelain from pineapple stem (EC 3.4.22.32), cellulase from Trichoderma reesei (EC 3.2.1.4), and viscozyme L from Aspergillus sp. (cellulolytic enzyme mixture) were purchased from Sigma-Aldrich (St. Louis, MO, USA) (Table 1 ). All other chemicals and reagents were of analytical grade and are listed as follows: sodium dodecyl sulfate, acrylamide, Coomassie brilliant blue, methanol, 1,2-bis (dimethylamino) ethane, 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), potassium persulfate, 2,2-diphenyl-1-picrylhydrazyl (DPPH), gallic acid, Folin–Ciocalteu’s phenol reagent, and sodium carbonate were acquired from Sigma-Aldrich. Trichloroacetic acid (TCA) was purchased from Samchun Chemical Co. (Pyeongtaek, Republic of Korea).
3
Bromelain from Pineapple Stem Preparation
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Bromelain from pineapple stem (B 4882 3 to 7 U/g) was purchased from Sigma Aldrich, Germany, prepared at a concentration of 10 caseinolitic units/mL in sodium phosphate buffer (877 mM NaH2PO4, 123 mM Na2HPO4, pH 6), and kept refrigerated at 4 °C until use.
4
Enzymatic Activity Assay Protocols
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High-performance liquid chromatography (HPLC)
gradel -aspartic acid diethyl ester hydrochloride (Et2-Asp) with 99.8% purity and α-chymotrypsin from bovine
pancreas with 94% protein were purchased from Chem-Impex International.l -Glutamic acid diethyl ester hydrochloride (Et2-Glu)
was purchased from TCI. Crude papain from Carica papaya (30 000 USP units/mg of solid) was purchased from Calbiochem
Co. Ltd. Trypsin from bovine pancreas and bromelain from pineapple
stem were purchased from Sigma-Aldrich. Potassium phosphate dibasic
trihydrate, casein, trichloroacetic acid, sodium carbonate anhydrous,
sodium acetate trihydrate, calcium acetate, andl -tyrosine
were purchased from Sigma-Aldrich. Folin & Ciocalteu’s
phenol reagent was purchased from MP Biomedicals. All the abovementioned
chemicals, reagents, and enzymes were obtained in the highest available
purity and were used as received.
grade
pancreas with 94% protein were purchased from Chem-Impex International.
was purchased from TCI. Crude papain from Carica papaya (30 000 USP units/mg of solid) was purchased from Calbiochem
Co. Ltd. Trypsin from bovine pancreas and bromelain from pineapple
stem were purchased from Sigma-Aldrich. Potassium phosphate dibasic
trihydrate, casein, trichloroacetic acid, sodium carbonate anhydrous,
sodium acetate trihydrate, calcium acetate, and
were purchased from Sigma-Aldrich. Folin & Ciocalteu’s
phenol reagent was purchased from MP Biomedicals. All the abovementioned
chemicals, reagents, and enzymes were obtained in the highest available
purity and were used as received.
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