Rat ria kit

Manufactured by Merck Group

Sourced in Germany

The Rat RIA kit is a laboratory equipment product designed for the quantitative determination of rat-specific proteins or hormones using the radioimmunoassay (RIA) technique. The kit provides the necessary reagents and components to perform RIA analysis on rat samples.

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RIA kit (51 citations) Rat insulin RIA kit (25 citations) Sensitive Rat Insulin RIA Kit (12 citations) GH Rat/Mouse Hormone RIA kit (2 citations) Rat specific insulin RIA Kit (2 citations)

4 protocols using rat ria kit

Insulin Secretion Capacity in Cells

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The capability of cells for insulin secretion was
measured using a rat RIA kit (Cat number: EZRMI-13K, Millipore, Germany). First, the cells of each group
were exposed to glucose-free Krebs-Ringer Bicarbonate
(KRB). Then, the cells were divided into three groups and
exposed to KRB containing different concentrations of
glucose 5.56, 16.7 and 25 mmol/L for one hour. Then, the
media were removed and kept in a -80°C freezer till use.
Finally, insulin secretion was measured by the rat RIA kit
(Cat number: EZRMI-13K, Millipore, Germany).

Hashemitabar M., Rezaei-Tazangi F., Khorsandi L, & Mard S.A. (2021). Autophagy Involves in Differentiation of Insulin-Secreting Cells from Adipose Derived Stem Cells. Cell Journal (Yakhteh), 23(6), 619-625.

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Glucose-Stimulated Insulin Secretion Assay

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The differentiated cells were pre-incubated
for one hour in glucose-free Krebs-ringer bicarbonate
(KRB) and incubated with KRB that
contained 5.56 mmol/L, 16.7 mmol/L or 25
mmol/L of glucose (glucose challenge) for an
additional one hour, respectively. The KRB media
were collected and frozen at -80˚C until assayed
(20 ). The insulin assay was performed by
radioimmunoassay (RIA) using a commercially
available rat RIA kit (Millipore) according to
the manufacturer’s instructions. Determinations
were carried out in triplicate and the means and
standard deviations were obtained.

Nejad-Dehbashi F., Hashemitabar M., Orazizadeh M., Bahramzadeh S., Shahhosseini Pourshoushtary E, & Khorsandi L. (2014). The Effects of Exendine-4 on Insulin Producing Cell Differentiation from Rat Bone Marrow-Derived Mesenchymal Stem Cells. Cell Journal (Yakhteh), 16(2), 187-194.

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Glucose-stimulated Insulin Secretion Assay

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The IPCs were incubated for 1 hour in glucose-free Krebs-Ringer bicarbonate (KRB). The cells were then incubated with KRB containing 5.56 mmol/L, 16.7 mmol/L and 25 mmol/L of glucose for 1 hour, respectively. The KRB media were collected and frozen at -80˚C until assay. Insulin assay was performed by RIA using a commercially available rat RIA kit (Millipore, Germany) according to the manufacturer’s instructions. Determinations were carried out in quadruplicate and the means and standard deviations were obtained.

Khorsandi L., Saremy S., Khodadadi A, & Dehbashi F. (2016). Effects of Exendine-4 on The Differentiation of Insulin Producing Cells from Rat Adipose-Derived Mesenchymal Stem Cells. Cell Journal (Yakhteh), 17(4), 720-729.

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Serum Metabolite Measurement Protocol

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Serum glucose, TAG, unesterified fatty acids (FFA), and total cholesterol were measured using colorimetric enzymatic assay kits from Wako Pure Chemicals (Osaka, Japan). Serum insulin was measured using a rat RIA kit from Millipore Corp. (Billerica, MA, USA).

Karahashi M., Hirata-Hanta Y., Kawabata K., Tsutsumi D., Kametani M., Takamatsu N., Sakamoto T., Yamazaki T., Asano S., Mitsumoto A., Kawashima Y, & Kudo N. (2016). Abnormalities in the Metabolism of Fatty Acids and Triacylglycerols in the Liver of the Goto-Kakizaki Rat: A Model for Non-Obese Type 2 Diabetes. Lipids, 51(8).

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