Luteolin (Lut,
>98%) was from Shanxi Huike Plant Co., Ltd. (Shanxi, China), and
bovine
serum albumin (BSA, ≥98%) was from Sigma Chemical Company (St.
Louis, USA). CuSO4·5H2O (>99%) and NaOH
(≥96%) were from Beijing Chemical Plant (Beijing, China). 2,2′-Azobis(2-methylpropionamidine)
dihydrochloride (AAPH, 98%) was from Energy Chemical (Anhui, China).
Warfarin and ibuprofen (≥98%) were from Aladdin Industrial
Co. (Shanghai, China). The 30% H2O2 (9.8 M)
aqueous solution (≥99.7%) was from Tianjin Fuchen Chemical
Reagent Factory (Tianjin, China). The buffer solution used in the
experiments was prepared using 3-(N-morpholino)propanesulfonic
acid (MOPS, ≥99.5%, Sigma-Aldrich, St. Louis, MO, USA). All
experiments were performed in a 25 mM MOPS buffer at pH 7.4. NaOH
was used to adjust the pH, and 3% (v/v) ethanol (99.9%, Fine Chemical
Industry Research Institute, Tianjin, China) was used to increase
the solubility of Lut. All experimental sections were repeated three
times in parallel to produce error analyses.
>98%) was from Shanxi Huike Plant Co., Ltd. (Shanxi, China), and
bovine
serum albumin (BSA, ≥98%) was from Sigma Chemical Company (St.
Louis, USA). CuSO4·5H2O (>99%) and NaOH
(≥96%) were from Beijing Chemical Plant (Beijing, China). 2,2′-Azobis(2-methylpropionamidine)
dihydrochloride (AAPH, 98%) was from Energy Chemical (Anhui, China).
Warfarin and ibuprofen (≥98%) were from Aladdin Industrial
Co. (Shanghai, China). The 30% H2O2 (9.8 M)
aqueous solution (≥99.7%) was from Tianjin Fuchen Chemical
Reagent Factory (Tianjin, China). The buffer solution used in the
experiments was prepared using 3-(N-morpholino)propanesulfonic
acid (MOPS, ≥99.5%, Sigma-Aldrich, St. Louis, MO, USA). All
experiments were performed in a 25 mM MOPS buffer at pH 7.4. NaOH
was used to adjust the pH, and 3% (v/v) ethanol (99.9%, Fine Chemical
Industry Research Institute, Tianjin, China) was used to increase
the solubility of Lut. All experimental sections were repeated three
times in parallel to produce error analyses.