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P akt ser473 t akt

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P‐Akt (Ser473)/t‐Akt is a lab equipment product that enables the detection and quantification of phosphorylated Akt (Ser473) and total Akt proteins in cellular samples. It is a tool for researchers to study cell signaling pathways involving the Akt kinase.

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Lab products found in correlation

Enhanced chemiluminescence reaction, by PerkinElmer (1 mentions) P erk thr202 tyr204 t erk, by Cell Signaling Technology (1 mentions) Bcat1, by Abcam (1 mentions) β actin, by Cell Signaling Technology (1 mentions) T egfr, by R&D Systems (1 mentions) P egfr, by Cell Signaling Technology (1 mentions) β actin 13e5, by Cell Signaling Technology (1 mentions) P axl, by Cell Signaling Technology (1 mentions)

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P-AKT (2452 citations) P-AKT (Ser473) (642 citations) T-AKT (82 citations) Akt/p-Akt (17 citations) (Ser473) Akt (6 citations) T-/p-AKT (3 citations)

2 protocols using p akt ser473 t akt

1

Western Blot Analysis of Cell Signaling

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Total cell lysates (20 µg) were resolved by 10% SDS‐PAGE, transferred to nitrocellulose membranes and immunoblotted with antibodies to BCAT1 (Abcam), Ras, phosphor (p)‐BRaf (Ser445)/total (t)‐BRaf, p‐MEK (Ser217/221)/t‐MEK, p‐ERK (Thr202/Tyr204)/t‐ERK and p‐Akt (Ser473)/t‐Akt (Cell Signaling). β‐Actin served as the loading control (Cell Signaling). Immunoreactive bands were identified using an enhanced chemiluminescence reaction (Perkin‐Elmer) and visualized by autoradiography.
Chen J., Barrett L., Lin Z., Kendrick S., Mu S., Dai L, & Qin Z. (2022). Identification of natural compounds tubercidin and lycorine HCl against small‐cell lung cancer and BCAT1 as a therapeutic target. Journal of Cellular and Molecular Medicine, 26(9), 2557-2565.
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2

Quantifying Protein Phosphorylation Signaling

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Western blotting was performed as described previously8 (link) using primary antibodies for p-AXL (Tyr702), t-AXL, p-EGFR (Tyr1086), β-actin (13E5), p-Akt (Ser473), t-Akt, p-Erk1/2 (Thr202/Tyr204), t-ERK1/2 (1:1000; Cell Signaling Technology), and t-EGFR (1:1000; R&D Systems).
Nakamura R., Fujii H., Yamada T., Matsui Y., Yaoi T., Honda M., Tanaka N., Miyagawa-Hayashino A., Yoshimura A., Morimoto K., Iwasaku M., Tokuda S., Kim Y.H., Konishi E., Itoh K, & Takayama K. (2023). Analysis of Tumor Heterogeneity Through AXL Activation in Primary Resistance to EGFR Tyrosine Kinase Inhibitors. JTO Clinical and Research Reports, 4(6), 100525.
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