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Stockert 70 radiofrequency generator

Manufactured by Johnson & Johnson
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The Stockert 70 is a radiofrequency generator manufactured by Johnson & Johnson. It is designed to generate radiofrequency energy for medical procedures. The core function of the Stockert 70 is to produce radiofrequency waves that can be used in various medical applications.

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3 protocols using stockert 70 radiofrequency generator

1

Standardized Cardiac Injury Model

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NHP-LV heart slices seeded with HVPs or CMs were injured on the opposite tissue border 3 days after seeding by applying 20 W for 15 s using a THERMOCOOL SF uni-directional catheter, tip electrode 3.5 mm (Biosense Webster) and Stockert 70 radiofrequency generator (Biosense Webster). During the RFA procedure, physiologic preload was reduced to 0.5 mN and re-adjusted to 1 mN after 2 days. For in vivo experiments, epicardial RFA with 25 W for 7 s was performed to produce a standardized, non-transmural injury.
Poch C.M., Foo K.S., De Angelis M.T., Jennbacken K., Santamaria G., Bähr A., Wang Q.D., Reiter F., Hornaschewitz N., Zawada D., Bozoglu T., My I., Meier A., Dorn T., Hege S., Lehtinen M.L., Tsoi Y.L., Hovdal D., Hyllner J., Schwarz S., Sudhop S., Jurisch V., Sini M., Fellows M.D., Cummings M., Clarke J., Baptista R., Eroglu E., Wolf E., Klymiuk N., Lu K., Tomasi R., Dendorfer A., Gaspari M., Parrotta E., Cuda G., Krane M., Sinnecker D., Hoppmann P., Kupatt C., Fritsche-Danielson R., Moretti A., Chien K.R, & Laugwitz K.L. (2022). Migratory and anti-fibrotic programmes define the regenerative potential of human cardiac progenitors. Nature Cell Biology, 24(5), 659-671.
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2

Radiofrequency Renal Denervation in Hypertensive Rats

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After baseline measurement of systemic cardiovascular function, SHRs were randomly divided into either an RF-RDN or a sham-RDN group. For these procedures, SHRs were anesthetized with isoflurane (2%), and a flank incision was made to expose the left renal artery. For RF-RDN, a segment (≈3 mm in length) of the proximal renal artery adjacent to the renal artery ostium (ie, bifurcation from the aorta) was carefully dissected, leaving any visible extravascular nerves intact. A small piece of plastic cut in the shape of a triangle was then placed under the renal artery as a platform and to protect the underlying tissue. The tip of the radiofrequency probe (6F, Celsius electrophysiology catheter) was then applied to 4-quadrants (circumferential) of the renal artery for 20 s each at 10 watts for the RF-RDN group or at 0 watts for the sham-RDN group (Stockert 70 radiofrequency generator and probes graciously provided by Biosense Webster). During RF-RDN, the temperature of the probe was not permitted to be higher than 65°C. After completion of the RF-RDN or sham-RDN procedure, the plastic was removed, and the muscle and skin were sutured closed in layers. The same RF-RDN or sham-RDN procedure was then performed on the contralateral renal artery.
Polhemus D.J., Gao J., Scarborough A.L., Trivedi R., McDonough K.H., Goodchild T.T., Smart F., Kapusta D.R, & Lefer D.J. (2016). Radiofrequency Renal Denervation Protects the Ischemic Heart via Inhibition of GRK2 and Increased Nitric Oxide Signaling. Circulation Research, 119(3), 470-480.
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3

Renal Denervation in Hypertensive Rats

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Following baseline measurement of systemic cardiovascular function, SHR were randomly divided into either a RF-RDN or sham-RDN group. For these procedures SHR were anesthetized with isoflurane (2 %) and a flank incision was made to expose the left renal artery. For RF-RDN, a segment (~3 mm in length) of the proximal renal artery adjacent to the renal artery ostium (i.e., bifurcation from the aorta) was carefully dissected leaving any visible extra-vascular nerves intact. A small piece of plastic cut in the shape of a triangle was then placed under the renal artery as a platform and to protect underlying tissue. The tip of the radiofrequency probe (6F, Celsius electrophysiology catheter) was then applied to 4-quadrants (circumferential) of the renal artery for 20s each at 10 Watts for the RF-RDN group or 0 Watts for the Sham-RDN group (Stockert 70 radiofrequency generator and probes graciously provided by Biosense Webster). During RF-RDN the temperature of the probe was not permitted to be higher than 65° C. Following completion of the RF-RDN or Sham-RDN procedure the plastic was removed and the muscle and skin were sutured closed in layers. The same RF-RDN or Sham-RDN procedure was then performed on the contralateral renal artery.
Polhemus D.J., Gao J., Scarborough A.L., Trivedi R., McDonough K.H., Goodchild T.T., Smart F., Kapusta D.R, & Lefer D.J. (2016). Radiofrequency Renal Denervation Protects the Ischemic Heart via Inhibition of GRK2 and Increased Nitric Oxide Signaling. Circulation Research, 119(3), 470-480.
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