Plasma concentrations of TXA in clinical samples were determined by ultra-high-performance liquid chromatography-tandem mass spectrometry. 4-aminocyclohexanecarboxylic acid was used as the internal standard with some modification compared to the published methods.26 (link),27 (link) Specifically, plasma samples were prepared for analysis using a protein precipitation process with acetonitrile. Sample extracts were analyzed using normal phase chromatography with a Waters BEH HILIC column (2.1*100 mm, 1.7 um, Waters Corp., Milford, MA, USA) followed by detection with a Waters Xevo TQ-XS mass spectrometer (Waters Corp., Milford, MA, USA). The lower limit of quantification for TXA was 0.04 μg/mL. Concentrations over 25 μg/mL were prepared first by dilution with blank plasma before analysis.
Waters xevo tq xs mass spectrometer
Manufactured by Waters Corporation
Sourced in United States, United Kingdom
The Waters Xevo TQ-XS mass spectrometer is a high-performance analytical instrument designed for quantitative and qualitative analysis. It utilizes tandem mass spectrometry technology to accurately measure the molecular mass and structure of chemical compounds.
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2 protocols using waters xevo tq xs mass spectrometer
1
Quantification of Tranexamic Acid in Plasma
2
Targeted Metabolic Profiling of Urine
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Urine samples were thawed at 4 °C and prepared for analyses following previously reported metabolic phenotyping methods [53] , with minor modifications. For the quantification of biogenic amines and amino acid metabolites, separation was performed by ultra-high-performance liquid chromatography (UHPLC) using an Acquity UPLC (Waters Corp, Milford, MA) coupled to a Bruker Impact II QToF mass spectrometer (Bruker, Daltonics, Billerica, MA). Resulting data files were processed for integrations and quantification using Target Analysis for Screening Quantification (TASQ) software v2.2 (Bruker, Daltonics, Bremen, Germany) where calibration curves were linearly fitted with a weighting factor 1/x. For the measurement of tryptophan and associated catabolites, separation was performed using Acquity UHPLC coupled to a Waters Xevo TQ-XS mass spectrometer (Waters Corp, Wilmslow, U.K.). Obtained raw files were processed for peak integrations and metabolite quantifications using the TargetLynx package within MassLynx v4.2, where calibration curves were linearly fitted for each metabolites using a weighting factor of 1/x. Resulting data matrices were quality controlled and combined prior to statistical analysis.
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