Hsp90

Manufactured by Beyotime

Sourced in China

Hsp90 is a molecular chaperone protein that plays a key role in maintaining the structural integrity and functional activity of various client proteins. It assists in the folding, stabilization, and activation of a diverse range of proteins involved in signal transduction, cell cycle regulation, and other cellular processes.

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Lab products found in correlation

2 protocols using hsp90

Tetrahydrocurcumin Modulates Epithelial-Mesenchymal Transition

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Tetrahydrocurcumin was purchased from Yuanye Biotechnology Co. Ltd (Shanghai,China). E-cadherin, ZEB, Snail, Twist, HIF-1α, p-mTOR, mTOR, p-ERK, ERK, GAPDH and β-actin antibodies were purchased from Santa cruz Biotechnology (Santa cruz, CA, USA). Vimentin, N-cadherin, VEGF, MMP-2, MMP-9, LaminA/C, Atg5, Atg7 and Beclin-1 antibodies were obtained from Boster Biological Technology Co. Ltd (Wuhan, China). LY294002, SB203580, Rapamycin, β-catenin, p-Akt, Akt, p-JNK, JNK, p-p38, p38, IκB-α, p65, Hsp90 and GSK-3β antibodies were obtained from Beyotime Institute of Biotechnology (Haimen, China). Antibody against LC3B was purchased from Abcam (Cambridge, UK). All other reagents were from common commercial sources.

Zhang Y., Liu Y., Zou J., Yan L., Du W., Zhang Y., Sun H., Lu P., Geng S., Gu R., Zhang H, & Bi Z. (2017). Tetrahydrocurcumin induces mesenchymal-epithelial transition and suppresses angiogenesis by targeting HIF-1α and autophagy in human osteosarcoma. Oncotarget, 8(53), 91134-91149.

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Western Blot Analysis of Mouse Liver Proteins

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Mouse liver protein was extracted by using RIPA buffer plus 1mM PMSF protein inhibitor (Beyotime Biotechnology, China). And 40μg protein/lane (liver tissue) and 10μg protein/lane (cell strain) were separated with 10% SDS-PAGE, and then was blotted to 0.2μm PVDF membrane (Millipore, MA, USA). After being blocked with 5% non-fat milk buffer (Yili Industrial Group Co., Ltd, China) for 1 h at room temperature, membrane was incubated with diluted Hsp90 (1:500, Beyotime, China), AKR7A (1:1000, Santa Cruz, USA), Bcl-2 (1:500, Beyotime, China), p44/42MAPK (1:1000, Beyotime, China), Actin (1:500, Beyotime, China) primary antibodies overnight at 4 °C, followed by HRP-coupled secondary antibody (1:2000, Beyotime, China) for 1h at room temperature. After incubating with chemiluminescent agent, the membrane was developed and imaged under Imager System (Bio-Rad, USA) [16] .

Wu K., Guo C., Su M., Wu X, & Li R. (2017). Biocharacterization of Heat Shock Protein 90 in Acetaminophen-Treated Livers Without Conspicuous Drug Induced Liver Injury. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 43(4).

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