Antibodies against smad2

Manufactured by Cell Signaling Technology

Sourced in United States

Antibodies against Smad2 are laboratory reagents used to detect and study the Smad2 protein, a key component of the TGF-beta signaling pathway. These antibodies can be used in various research applications, such as Western blotting, immunoprecipitation, and immunohistochemistry.

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Lab products found in correlation

P smad2, by Cell Signaling Technology (2 mentions) P smad3, by Cell Signaling Technology (2 mentions) Supersignal west femto kit, by Thermo Fisher Scientific (1 mentions) Recombinant human tgf β3, by R&D Systems (1 mentions) Recombinant human bmp 2, by Thermo Fisher Scientific (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Anti β actin antibody, by Merck Group (1 mentions) Igf 1, by Abcam (1 mentions) Ecl chemiluminescence kit, by Applygen (1 mentions) 8 ohdg, by Abcam (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) α sma, by Abcam (1 mentions)

Spelling variants of this product

Smad2 (224 citations) Antibodies against Smad2/3 (2 citations) Antibodies against phospho-smad2/3 (2 citations) Antibodies against p-Smad2/3 (2 citations) Antibodies against p-Smad2 (2 citations)

3 protocols using antibodies against smad2

Western Blot Analysis of TGF-β Signaling Pathway

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Cells were lysed in a denaturing SDS sample buffer. Proteins in the cell lysates were separated on SDS/10-15% polyacrylamide gels and transferred to Immobilon-P membranes. The membranes were pre-incubated with 5% skim milk for 30 min, incubated overnight with primary antibodies (1:1000 dilution) at 4 °C, and incubated with HRP–conjugated secondary antibodies (1:5000) at room temperature for 1 h. The immune complexes were visualized using the SuperSignal West Femto kit (Thermo Scientific, Waltham, MA). Antibodies against Smad2, p-Smad2, Smad3, and p-Smad3 were purchased from Cell Signaling Tech. (Danvers, MA); TGFβR1 antibody from Santa Cruz Biotech. (Dallas, TX); α-SMA, collagen-1α, and p-TGFβR1 antibodies from Abcam (Cambridge, MA); CST3 antibody from R&D Systems (Minneapolis, MN); GDF15 antibody from Fisher Scientific (Pittsburgh, PA).

Kim Y.I., Shin H.W., Chun Y.S., Cho C.H., Koh J., Chung D.H, & Park J.W. (2018). Epithelial cell-derived cytokines CST3 and GDF15 as potential therapeutics for pulmonary fibrosis. Cell Death & Disease, 9(5), 506.

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Chondrocyte Regulatory Pathway Analysis

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Recombinant human TGF-β3 was purchased from R&D systems (Minneapolis, MN, USA). Recombinant human BMP-2 was purchased from PeproTech (Rocky Hill, NJ, USA). Antibodies against lubricin and glucocorticoid receptor (GR: phosphorylated-GR-S211 and total) were purchased from Abcam (Cambridge, UK). Antibodies against Smad-2, Smad-3, AKT, ribosomal protein p70S6k and their respective phosphorylated epitopes were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA). Antibodies against SOX9 and collagen type II were purchased from Chemicon (Billerica, MA, USA). Anti-β-actin antibody and dexamethasone (DEX) were purchased from Sigma (St Louis, MO, USA). An FDA-approved drug library and orphan ligand drug library were purchased from Enzo Life Sciences (Tokyo, Japan). Fluocinolone acetonide (FA), triamcinolone acetonide (TA) and mifepristone/RU486 were purchased from Tokyo Chemical Industry Inc. (Tokyo, Japan). Rapamycin and AKT1/2 inhibitor vii were purchased from Calbiochem (San Diego, CA).

Hara E.S., Ono M., Hai P.T., Sonoyama W., Kubota S., Takigawa M., Matsumoto T., Young M.F., Olsen B.R, & Kuboki T. (2015). Fluocinolone acetonide is a potent synergistic factor of TGF-β3-associated chondrogenesis of bone marrow-derived mesenchymal stem cells for articular surface regeneration. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 30(9), 1585-1596.

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Evaluating Fibrosis Biomarkers in TGF-β1 Model

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Antibodies against TGF-β1, 8-OHdG, α-SMA, S100A4, and IGF1 were obtained from Abcam Biotechnology Company (Cambridge, UK). Antibodies against Smad2, p-Smad2, Smad3, p-Smad3, and cleaved-caspase3 were purchased from Cell Signaling Technology (Denver, CO). UA was purchased from Wanxiang Hengyuan Biotechnology (Tianjin, China). Masson and Alcian blue-periodic acid Schiff (AB-PAS) kits were obtained from Nanjing Jiancheng biological engineering research institute (Nanjing, China). BCA kit was obtained from Pierce (Thermo-Scientific, Rockford, IL, USA), ECL chemiluminescence kit from Applygen (Beijing, China).

Lin L., Hou G., Han D., Yin Y., Kang J, & Wang Q. (2019). Ursolic acid alleviates airway-vessel remodeling and muscle consumption in cigarette smoke-induced emphysema rats. BMC Pulmonary Medicine, 19, 103.

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