The cerebral cortex neurons of E15 mice were cultured, as described previously [4 (link), 6 (link), 21 (link)]. For the inhibition assay of MAP 1B phosphorylation, 4 days in vitro (DIV) cortical neurons were treated for 24 h with culture medium containing DMSO as a control, one of three JNK1 inhibitors [SP600125, JNK Inhibitor V, and JNK Inhibitor XVI (Cayman Chemical, USA)], or one of three GSK3β inhibitors [IM-12, TDZD-8 (Cayman Chemical, USA), and LiCl (99.5% purity, Wako, Osaka, Japan)]. Cells exposed to each inhibitor were lysed in 1 × SDS-PAGE sample buffer and processed for western blotting analysis.
Jnk inhibitor 5
Manufactured by Cayman Chemical
Sourced in United States
JNK inhibitor V is a small molecule compound that selectively inhibits the c-Jun N-terminal kinase (JNK) signaling pathway. JNK inhibitors are a class of pharmacological agents used in research to study the role of the JNK pathway in cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Pitstop 2, by Merck Group (2 mentions)
Sb203580, by Enzo Life Sciences (2 mentions)
Lps rs, by InvivoGen (2 mentions)
Il 1r antagonist, by Cayman Chemical (2 mentions)
Ikk 16, by Cayman Chemical (2 mentions)
Dynasore hydrate, by Merck Group (2 mentions)
Cu cpt22, by Merck Group (2 mentions)
U0126, by Cell Signaling Technology (2 mentions)
Sp600125, by Cayman Chemical (1 mentions)
Lithium chloride (licl), by Fujifilm (1 mentions)
Tdzd 8, by Cayman Chemical (1 mentions)
3 protocols using jnk inhibitor 5
1
Inhibition of MAP1B Phosphorylation in Cortical Neurons
2
Elucidating Signaling Pathways Modulating NP Effects
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The antagonists or inhibitors involved in the NF-κB and MAPK signal pathways were introduced to verify the action of NP. The endocytosis inhibitors Pitstop2 (12.5 μM, Sigma) and Dynasore hydrate (12.5 μM, Sigma), the TLR4 antagonist LPS-RS (10 μg/ml; InvivoGen, San Diego, CA), the TLR1/TLR2 antagonist CU-CPT22 (20 μM, Millipore, Burlington, MA), the IL-1R antagonist (20 μM; Cayman Chemical Company), IKK-16 (20 μM; Cayman Chemical Company), the JNK inhibitor V (20 μM; Cayman Chemical Company), the ERK1/2 inhibitor U0126 (20 μM, Cell Signal Technology), and the p38 inhibitor SB203580 (20 μM; Enzo Life Sciences, Farmingdale, NY) were added to HLMEC cultures 1 h before NP exposure. The whole-cell lysates were harvested 8 h after NP stimulation and analyzed by Western blotting.
3
Deciphering NF-κB and MAPK Signaling Pathways
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The antagonists or inhibitors involved in NF-κB and MAPK signal pathway were introduced to verify the action of NP. The endocytosis inhibitors Pitstop 2 (12.5 μM, Sigma) and Dynasore hydrate (12.5 μM, Sigma), the TLR4 antagonist LPS-RS (10 μg/mL, InVivoGen, San Diego, CA), the TLR1/TLR2 antagonist CU-CPT22 (20 μM, Millipore, Burlington, MA), the IL-1R antagonist (20 μM, Cayman Chemical Company), IKK-16 (20 μM, Cayman Chemical Company), the JNK inhibitor V (20 μM, Cayman Chemical Company), the ERK1/2 inhibitor U0126 (20 μM, Cell Signal Technology), and the p38 inhibitor SB203580 (20 μM, Enzo Life Sciences, Farmingdale, NY) were added into HLMEC cultures 1 h before NP exposure. The whole cell lysates were harvested 8 h after NP stimulation and analyzed by western blot.
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