Sasi hs01 00126206

Manufactured by Merck Group

SASI_Hs01_00126206 is a lab equipment product from Merck Group. It is a device used for scientific research and analysis. The core function of this product is to perform specific tasks related to laboratory experimentation and data collection. No further details can be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions) Sic002, by Merck Group (1 mentions) Sasi hs01 00220872, by Merck Group (1 mentions) Transit x2, by Mirus Bio (1 mentions) Sic001, by Merck Group (1 mentions) Memerald atg5 c 18, by Addgene (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)

2 protocols using sasi hs01 00126206

Knockdown of Myo5A, Myo5B, RAB11A, RAB11B

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Cells were transfected with 20 nM of Myo5A predesigned siRNA (SASI_Hs01_00181251; Sigma Aldrich), Myo5B predesigned siRNA (SASI_Hs01_00220872; Sigma Aldrich), RAB11A predesigned siRNA (SASI_Hs01_00126206; Sigma Aldrich), RAB11B predesigned siRNA (SASI_Hs01_00220872; Sigma Aldrich) or Negative control siRNA (SIC002; Sigma Aldrich) for 5 h at 37 °C in SFM using Lipofectamine RNAiMAX (Thermo Fisher Scientific), then were maintained in LHC8 complete medium and used in the following experiments.

Sakai T., Choo Y.Y., Sato O., Ikebe R., Jeffers A., Idell S., Tucker T, & Ikebe M. (2022). Myo5b Transports Fibronectin-Containing Vesicles and Facilitates FN1 Secretion from Human Pleural Mesothelial Cells. International Journal of Molecular Sciences, 23(9), 4823.

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Plasmid constructs for HBV protein expression

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The plasmid p1.3xHBV contains the overlength 1.3mer HBV genomic DNA in the pUC19 vector (51 (link)). p1.3xHBV PC^– (PC MT), and p1.3xHBV S^– (S MT) contained 1.3mer HBV genome with mutations that abolished the expression of the HBV precore protein and HBsAg, respectively. The S MT was constructed by mutating nucleotide 223 from T to A to generate a premature termination codon. This mutation did not affect the overlapping polymerase coding sequence. HBV protein expression plasmids, including pCMV-precore, pCMV-core, CM1, and CM2 had been described before (36 (link)). The plasmid pcDNA-p22 was constructed by deleting the signal peptide-coding sequence (amino acids 2 to 19) from pCMV-precore. The DNA plasmid mEmerald-ATG5-C-18 was purchased from Addgene (Addgene plasmid #54000). siRNA targeting human Rab11a (SASI_Hs01_00126206) and a negative control siRNA (SIC001) were purchased from Sigma-Aldrich. The DNA and siRNA transfections were performed using Lipofectamine 3000 (Invitrogen) and TransIT-X2 (Mirus) per the manufacturer’s instructions.

Chu J.Y., Chuang Y.C., Tsai K.N., Pantuso J., Ishida Y., Saito T, & Ou J.H. (2022). Autophagic membranes participate in hepatitis B virus nucleocapsid assembly, precore and core protein trafficking, and viral release. Proceedings of the National Academy of Sciences of the United States of America, 119(30), e2201927119.

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