Csfv ab elisa

For antibody detection against CSFV, two commercially available ELISAs were carried out according to the manufactures instructions. The antibody ELISAs are different with regard to antigen specificity, the CSFV Ab ELISA (IDEXX) detects antibodies against the structural glycoprotein E2 and the pigtype^® CSFV E^rns AB ELISA (Indicial) against the structural protein E^rns.

The protocol for inactivation was validated using a panel consisting of 33 serum samples with different virological and serological properties. To confirm virus inactivation, the samples were treated with PBS 0.3% Tween₂₀ and heat (56 °C for 30 min), and thereafter subjected to virus isolation. Subsequently, serological analysis (VNT, CSFV Ab ELISA (IDEXX) and pigtype^® CSF E^rns AB ELISA (Indicial)) were performed. To consider a safety margin regarding virus inactivation, the samples were analyzed after treatment with PBS 0.5% Tween₂₀ and also heat. For comparison, untreated serum samples were also analyzed.

To determine the interference with the serological CSFV diagnostic, a commercially available CSFV-specific antibody ELISA (CSFV Ab ELISA; IDEXX) was used for the analysis of the experimentally derived serum samples. The protocol follows the manufacturers´ instructions.