Primary antibodies used are as follows: chicken polyclonal anti-GFP (1:1000, Abcam, MA), rabbit polyclonal anti-GFP (1:1000, Invitrogen, NY), rabbit polyclonal anti-UCHL1 (1:1000, Proteintech, IL), chicken polyclonal anti-neurofilament-heavy (NF-H, 1:1000, Millipore, MA), rabbit polyclonal anti-parvalbumin (PV, 1:5000, Swant, Switzerland), rabbit polyclonal anti-calcitonin gene-related peptide (CGRP, 1:1000, Chemicon, MA). Far red-conjugated isolectin B4 (IB4, 1:500, Invitrogen, NY) was included with anti-GFP primary antibody. Appropriate secondary fluorescent antibodies (1:1000, AlexaFluor-488-conjugated, Invitrogen, NY; FITC-conjugated, Invitrogen, NY; or Cy3-conjugated, Millipore, MA) were added to blocking solution at room temperature for 2 h in the dark. All antibodies used were characterized previously, and specificity validated by using controls processed alongside other samples omitting the primary antibodies. GFP immunocytochemistry was further validated by using WT mouse tissue that does not contain the eGFP transgene.
Rabbit polyclonal anti parvalbumin pv
Manufactured by Swant
Sourced in Greece
Rabbit polyclonal anti-parvalbumin (PV) is a laboratory reagent used to detect the presence and distribution of parvalbumin, a calcium-binding protein, in biological samples. It is produced by immunizing rabbits with purified parvalbumin and collecting the resulting antibodies.
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Lab products found in correlation
Rabbit polyclonal anti gfp, by Thermo Fisher Scientific (1 mentions)
Chicken polyclonal anti gfp, by Abcam (1 mentions)
Tcs sp2, by Leica (1 mentions)
Alexa fluor 488 goat anti rabbit, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 555 goat anti rabbit, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 goat anti rat, by Thermo Fisher Scientific (1 mentions)
Rat monoclonal anti gfp, by Nacalai Tesque (1 mentions)
2 protocols using rabbit polyclonal anti parvalbumin pv
1
Immunostaining of Neuronal Markers
2
Immunolabeling of Prefrontal Cortex Neurons
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Mice at the age of P10 and P20 were perfused with 4% paraformaldehyde, followed by fixation with the same solution for 1h at 4•C, followed by cryoprotection and preparation of 12 μm cryostat sections as previously described (Vidaki et al., 2012) . Primary antibodies used were rat monoclonal anti-GFP (Nacalai Tesque, Kyoto, Japan, 1:5000), rabbit polyclonal anti-GFP (1:500; Minotech Biotechnology, Heraklion, Greece) and rabbit polyclonal anti-parvalbumin (PV) (Swant, Bellinzona, Switzerland; 1:2000. Secondary antibodies used were goat anti-rat-Alexa Fluor-488, goat anti-rabbit Alexa Fluor-488, and goat anti-rabbit-Alexa Fluor-555 (Molecular Probes, Eugene, OR, United States, 1:800). Images were obtained with a confocal microscope (Leica TCS SP2, Leica, Nussloch, Germany). For each age group (P10, P20), 2-4 10μm-thick sections from each mouse brain were selected, all including the mPFC and BC.
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